Abstract
This report describes a procedure by which high performance liquid chromatographic (HPLC) techniques are used to obain homogeneous S-antigen. Conventionally prepared S-antigen was purified further by fractionation on the anion exchange Mono-Q column followed by gel filtration on a TSK-3000 columm. This procedure produced an S-antigen preparation, which appeared homogeneous by gel electrophoresis using the very sensitive silver staining method. The purified material retained its immunochemical and uveitogenic activity. The availability of homogeneous S-antigen will facilitate studies aimed at elucidating, at the molecular level, the mechanism by which S-antigen induces uveitis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 977-980 |
| Number of pages | 4 |
| Journal | Investigative Ophthalmology and Visual Science |
| Volume | 25 |
| Issue number | 8 |
| State | Published - Jan 1 1984 |
ASJC Scopus subject areas
- Ophthalmology
- Sensory Systems
- Cellular and Molecular Neuroscience