This report describes a procedure by which high performance liquid chromatographic (HPLC) techniques are used to obain homogeneous S-antigen. Conventionally prepared S-antigen was purified further by fractionation on the anion exchange Mono-Q column followed by gel filtration on a TSK-3000 columm. This procedure produced an S-antigen preparation, which appeared homogeneous by gel electrophoresis using the very sensitive silver staining method. The purified material retained its immunochemical and uveitogenic activity. The availability of homogeneous S-antigen will facilitate studies aimed at elucidating, at the molecular level, the mechanism by which S-antigen induces uveitis.
|Original language||English (US)|
|Number of pages||4|
|Journal||Investigative Ophthalmology and Visual Science|
|State||Published - Jan 1 1984|
ASJC Scopus subject areas
- Sensory Systems
- Cellular and Molecular Neuroscience