TY - JOUR
T1 - Purification of heat-labile enterotoxin from four Escherichia coli strains by affinity immunoadsorbent
T2 - Evidence for similar subunit structure
AU - Dafni, Z.
AU - Sack, R. B.
AU - Craig, J. P.
PY - 1978
Y1 - 1978
N2 - A single-step method for the purification of the heat-labile enterotoxin of E. coli is described. The method involves an affinity immunoadsorbent made with antiserum to cholera toxin. Crude toxin preparations of three human and one porcine enterotoxinogenic strains of E. coli were purified on this immunoadsorbent, and the elution products suggest that the toxin molecule is composed of subunits. One kind of subunit shared by these four strains showed similar mobility of sodium dodecyl sulfate-polyacrylamide gel electrophoresis, close antigenic relationship, and an antigen in common with cholera enterotoxin.
AB - A single-step method for the purification of the heat-labile enterotoxin of E. coli is described. The method involves an affinity immunoadsorbent made with antiserum to cholera toxin. Crude toxin preparations of three human and one porcine enterotoxinogenic strains of E. coli were purified on this immunoadsorbent, and the elution products suggest that the toxin molecule is composed of subunits. One kind of subunit shared by these four strains showed similar mobility of sodium dodecyl sulfate-polyacrylamide gel electrophoresis, close antigenic relationship, and an antigen in common with cholera enterotoxin.
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U2 - 10.1128/iai.22.3.852-860.1978
DO - 10.1128/iai.22.3.852-860.1978
M3 - Article
C2 - 103838
AN - SCOPUS:0018246039
SN - 0019-9567
VL - 22
SP - 852
EP - 860
JO - Infection and immunity
JF - Infection and immunity
IS - 3
ER -