Purification of Giardia muris trophozoites by using nylon fiber columns

J. S. Andrews; J. J. Ellner; D. P. Stevens

Purification of Giardia muris trophozoites by using nylon fiber columns

J. S. Andrews, J. J. Ellner, D. P. Stevens

Research output: Contribution to journal › Article › peer-review

Abstract

A method is described for purification of large quantities of Giardia muris trophozoites from the small intestine of the mouse by using density layer centrifugation and nylon fiber columns. Centrifugation of trophozoites obtained from the small intestine on Metrizamide®, specific gravity 1.10, followed by incubation on a nylon fiber column at 37°C for 120 min, yielded up to 15 x 10⁶ viable purified trophozoites per infected mouse.

Original language	English (US)
Pages (from-to)	12-15
Number of pages	4
Journal	American Journal of Tropical Medicine and Hygiene
Volume	29
Issue number	1
State	Published - 1980
Externally published	Yes

ASJC Scopus subject areas

Parasitology
Infectious Diseases

Cite this

@article{972c3188d2c64a7d836ffb62188d800a,

title = "Purification of Giardia muris trophozoites by using nylon fiber columns",

abstract = "A method is described for purification of large quantities of Giardia muris trophozoites from the small intestine of the mouse by using density layer centrifugation and nylon fiber columns. Centrifugation of trophozoites obtained from the small intestine on Metrizamide{\textregistered}, specific gravity 1.10, followed by incubation on a nylon fiber column at 37°C for 120 min, yielded up to 15 x 106 viable purified trophozoites per infected mouse.",

author = "Andrews, {J. S.} and Ellner, {J. J.} and Stevens, {D. P.}",

year = "1980",

language = "English (US)",

volume = "29",

pages = "12--15",

journal = "American Journal of Tropical Medicine and Hygiene",

issn = "0002-9637",

publisher = "American Society of Tropical Medicine and Hygiene",

number = "1",

}

TY - JOUR

T1 - Purification of Giardia muris trophozoites by using nylon fiber columns

AU - Andrews, J. S.

AU - Ellner, J. J.

AU - Stevens, D. P.

PY - 1980

Y1 - 1980

N2 - A method is described for purification of large quantities of Giardia muris trophozoites from the small intestine of the mouse by using density layer centrifugation and nylon fiber columns. Centrifugation of trophozoites obtained from the small intestine on Metrizamide®, specific gravity 1.10, followed by incubation on a nylon fiber column at 37°C for 120 min, yielded up to 15 x 106 viable purified trophozoites per infected mouse.

AB - A method is described for purification of large quantities of Giardia muris trophozoites from the small intestine of the mouse by using density layer centrifugation and nylon fiber columns. Centrifugation of trophozoites obtained from the small intestine on Metrizamide®, specific gravity 1.10, followed by incubation on a nylon fiber column at 37°C for 120 min, yielded up to 15 x 106 viable purified trophozoites per infected mouse.

UR - http://www.scopus.com/inward/record.url?scp=0018914416&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018914416&partnerID=8YFLogxK

M3 - Article

C2 - 7352620

AN - SCOPUS:0018914416

SN - 0002-9637

VL - 29

SP - 12

EP - 15

JO - American Journal of Tropical Medicine and Hygiene

JF - American Journal of Tropical Medicine and Hygiene

IS - 1

ER -

Purification of Giardia muris trophozoites by using nylon fiber columns

Abstract

ASJC Scopus subject areas

Other files and links

Fingerprint

Cite this