Purification and properties of a collagenase inhibitor from cultures of bovine aorta

Joseph C. Nolan; Susan C. Ridge; Arnold L. Oronsky; S. S. Kerwar

doi:10.1016/0021-9150(80)90031-3

Purification and properties of a collagenase inhibitor from cultures of bovine aorta

Joseph C. Nolan, Susan C. Ridge, Arnold L. Oronsky, S. S. Kerwar

School of Medicine

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Bovine medial explants in culture synthesize a potent inhibitor of mammalian collagenase but not of bacterial collagenase. This inhibitor has been partially purified and has an apparent molecular weight of 45,000. It is a glycoprotein pro is stable to heat, trypsin, acid and mercurials. Inhibitory activity is destroyed on reductive alkylation. The inhibitor interacts with collagenase and this interaction leads to the loss of enzymatic activity. This inhibitor may play a physiological role in the control of collagen degradation in blood vessels.

Original language	English (US)
Pages (from-to)	93-102
Number of pages	10
Journal	Atherosclerosis
Volume	35
Issue number	1
DOIs	https://doi.org/10.1016/0021-9150(80)90031-3
State	Published - Jan 1980

Keywords

Atherosclerosis
Collagen turnover
Collagenase
Collagenase inhibitor

ASJC Scopus subject areas

Cardiology and Cardiovascular Medicine

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10.1016/0021-9150(80)90031-3

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abstract = "Bovine medial explants in culture synthesize a potent inhibitor of mammalian collagenase but not of bacterial collagenase. This inhibitor has been partially purified and has an apparent molecular weight of 45,000. It is a glycoprotein pro is stable to heat, trypsin, acid and mercurials. Inhibitory activity is destroyed on reductive alkylation. The inhibitor interacts with collagenase and this interaction leads to the loss of enzymatic activity. This inhibitor may play a physiological role in the control of collagen degradation in blood vessels.",

keywords = "Atherosclerosis, Collagen turnover, Collagenase, Collagenase inhibitor",

author = "Nolan, {Joseph C.} and Ridge, {Susan C.} and Oronsky, {Arnold L.} and Kerwar, {S. S.}",

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AU - Nolan, Joseph C.

AU - Ridge, Susan C.

AU - Oronsky, Arnold L.

AU - Kerwar, S. S.

PY - 1980/1

Y1 - 1980/1

N2 - Bovine medial explants in culture synthesize a potent inhibitor of mammalian collagenase but not of bacterial collagenase. This inhibitor has been partially purified and has an apparent molecular weight of 45,000. It is a glycoprotein pro is stable to heat, trypsin, acid and mercurials. Inhibitory activity is destroyed on reductive alkylation. The inhibitor interacts with collagenase and this interaction leads to the loss of enzymatic activity. This inhibitor may play a physiological role in the control of collagen degradation in blood vessels.

AB - Bovine medial explants in culture synthesize a potent inhibitor of mammalian collagenase but not of bacterial collagenase. This inhibitor has been partially purified and has an apparent molecular weight of 45,000. It is a glycoprotein pro is stable to heat, trypsin, acid and mercurials. Inhibitory activity is destroyed on reductive alkylation. The inhibitor interacts with collagenase and this interaction leads to the loss of enzymatic activity. This inhibitor may play a physiological role in the control of collagen degradation in blood vessels.

KW - Atherosclerosis

KW - Collagen turnover

KW - Collagenase

KW - Collagenase inhibitor

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JF - Atherosclerosis

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ER -

Purification and properties of a collagenase inhibitor from cultures of bovine aorta

Abstract

Keywords

ASJC Scopus subject areas

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