@inbook{f8306a5055514d64b676afe187becfd2,
title = "Purification and immune phenotyping of B-1 cells from body cavities of mice",
abstract = "B-1 cells are fetal-origin B lymphocytes with unique developmental and functional characteristics that can generate natural, polyreactive antibodies with important functions in tissue homeostasis and immune defense. While B-1 cell frequencies in bone marrow and secondary lymphoid tissues are low, relative high frequencies exist within peritoneal and pleural cavities of mice, including both CD5+ and CD5− B-1 cells. These cells represent B-1 reservoirs that, when activated, migrate to lymphoid tissues to secrete antibodies and/or cytokines. Here, we outline efficient methods for the extraction and magnetic isolation of CD5+ B-1 cells from the peritoneal and pleural cavities as well as the separation and phenotypic characterization of CD5+ and CD5− B-1 cells by flow cytometry.",
keywords = "B-1 cell isolation, B-cell isolation, B-cell subsets, Innate-like B cells, Natural antibodies, Peritoneal cavity",
author = "Vanessa Yenson and Nicole Baumgarth",
note = "Funding Information: Data for the B-1 cell FACS-sorts were kindly provided by Dr. Elizabeth E. Waffarn (Columbia University). This work was supported in part by NIH U19AI109962 and AI117890. Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2021.",
year = "2021",
doi = "10.1007/978-1-0716-1237-8\_2",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "27--45",
booktitle = "Methods in Molecular Biology",
}