@inbook{8060e91ace184576b1b841ffe195fd2e,
title = "Purification and immune phenotyping of B-1 cells from body cavities of mice",
abstract = "B-1 cells are innate-like lymphocytes that generate natural, polyreactive antibodies with important functions in tissue homeostasis and immune defense. While B-1-cell frequencies in secondary lymphoid tissues are low, relative high frequencies are found within peritoneal and pleural cavities of mice, including both CD5+ B-1a and CD5- B-1b cells. They represent reservoirs of B-1 cells that can be activated for migration to lymphoid tissues to secrete antibodies and/or cytokines. Here, we outline efficient methods for the extraction and magnetic isolation of B-1a cells from the peritoneal and pleural cavities and the separation and phenotypic characterization of B-1a and B1-b cells by flow cytometry.",
keywords = "B-1-cell isolation, B-cell isolation, B-cell subsets, Innate-like B cells, Natural antibodies, Peritoneal cavity",
author = "Vanessa Yenson and Nicole Baumgarth",
year = "2014",
doi = "10.1007/978-1-4939-1161-5_2",
language = "English (US)",
isbn = "9781493911608",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "17--34",
booktitle = "Regulatory B Cells",
}