Purification and immune phenotyping of B-1 cells from body cavities of mice

Vanessa Yenson, Nicole Baumgarth

Research output: Chapter in Book/Report/Conference proceedingChapter


B-1 cells are innate-like lymphocytes that generate natural, polyreactive antibodies with important functions in tissue homeostasis and immune defense. While B-1-cell frequencies in secondary lymphoid tissues are low, relative high frequencies are found within peritoneal and pleural cavities of mice, including both CD5+ B-1a and CD5- B-1b cells. They represent reservoirs of B-1 cells that can be activated for migration to lymphoid tissues to secrete antibodies and/or cytokines. Here, we outline efficient methods for the extraction and magnetic isolation of B-1a cells from the peritoneal and pleural cavities and the separation and phenotypic characterization of B-1a and B1-b cells by flow cytometry.

Original languageEnglish (US)
Title of host publicationRegulatory B Cells
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Number of pages18
ISBN (Print)9781493911608
StatePublished - 2014
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • B-1-cell isolation
  • B-cell isolation
  • B-cell subsets
  • Innate-like B cells
  • Natural antibodies
  • Peritoneal cavity

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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