Provirus DNA sequences in Rous sarcoma cell genome

E. A. Ratovitski, L. P. Korobitsyn, J. D. Shaposhnikov, J. F. Seitz

Research output: Contribution to journalArticlepeer-review


Non-producer hamster and virus-producing chicken Rous sarcoma cells contain a complete avian sarcoma virus (ASV)-provirus DNA (pro-DNA). Unlike this, in normal hamster liver DNA the ASV-specific sequences are absent. Moreover, the nuclear chicken Rous sarcoma and chick embryo cell (CEC) DNA preparations contain practically all endogenous chicken virus (ECV)-specific sequences, while the hamster tumor DNA was annealed with only more than half of the ECV-RNA sequences. Thus, the pro-DNA of permissive hosts contains so-called 'sarcoma-specific', 'common' and 'endogenous-specific' nucleotide sequences. The 'sarcoma-specific' and 'common' sequences are present in the hamster sarcoma pro-DNA, only. Both the pro-DNA of permissive cells and the pro-DNA of non-permissive cells consist of moderately reiterated and unique sequences. The 'common' regions of pro-DNA are localized, mainly, in the unique zone, while 'sarcoma-specific' and 'endogenous-specific' sequences of pro-DNA - in the moderately reiterated and unique zones. The pro-DNA organization of permissive and non-permissive hosts is discussed.

Original languageEnglish (US)
Pages (from-to)497-507
Number of pages11
Issue number5
StatePublished - 1980
Externally publishedYes

ASJC Scopus subject areas

  • Cancer Research


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