Non-producer hamster and virus-producing chicken Rous sarcoma cells contain a complete avian sarcoma virus (ASV)-provirus DNA (pro-DNA). Unlike this, in normal hamster liver DNA the ASV-specific sequences are absent. Moreover, the nuclear chicken Rous sarcoma and chick embryo cell (CEC) DNA preparations contain practically all endogenous chicken virus (ECV)-specific sequences, while the hamster tumor DNA was annealed with only more than half of the ECV-RNA sequences. Thus, the pro-DNA of permissive hosts contains so-called 'sarcoma-specific', 'common' and 'endogenous-specific' nucleotide sequences. The 'sarcoma-specific' and 'common' sequences are present in the hamster sarcoma pro-DNA, only. Both the pro-DNA of permissive cells and the pro-DNA of non-permissive cells consist of moderately reiterated and unique sequences. The 'common' regions of pro-DNA are localized, mainly, in the unique zone, while 'sarcoma-specific' and 'endogenous-specific' sequences of pro-DNA - in the moderately reiterated and unique zones. The pro-DNA organization of permissive and non-permissive hosts is discussed.
|Original language||English (US)|
|Number of pages||11|
|State||Published - 1980|
ASJC Scopus subject areas
- Cancer Research