Proton magnetic resonance spectroscopy of skeletal muscle: A comparison of two quantitation techniques

Xin Wang, Nouha Salibi, Laura M. Fayad, Peter B. Barker

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


Rationale and objectives The aim of this study was to develop and compare two methods for quantification of metabolite concentrations in human skeletal muscle using phased-array receiver coils at 3 T. Materials and methods Water suppressed and un-suppressed spectra were recorded from the quadriceps muscle (vastus medialis) in 8 healthy adult volunteers, and from a calibration phantom containing 69 mM/L N-acetyl aspartate. Using the phantom replacement technique, trimethylamine specifically [TMA] and creatine [Cr] concentrations were estimated, and compared to those values obtained by using the water reference method. Results Quadriceps [TMA] concentrations were 9.5 ± 2.4 and 9.6 ± 4.1 mmol/kg wet weight using the phantom replacement and water referencing methods respectively, while [Cr] concentrations were 26.8 ± 12.2 and 24.1 ± 5.3 mmol/kg wet weight respectively. Conclusions Reasonable agreement between water referencing and phantom replacement methods was found, although for [Cr] variation was significantly higher for the phantom replacement technique. The relative advantages and disadvantages of each approach are discussed.

Original languageEnglish (US)
Pages (from-to)81-84
Number of pages4
JournalJournal of Magnetic Resonance
StatePublished - Jun 2014


  • MR spectroscopy
  • Muscle
  • Phantom replacement
  • Quantitation

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Nuclear and High Energy Physics
  • Condensed Matter Physics


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