@inbook{93b6a1fd8f5e452abe6f8b79227a67f9,
title = "Protocol for the Investigation of Plasma and Whole Blood Clot Property of Fibrin Fiber Thickness Using Scanning Electron Microscopy",
abstract = "Blood clot formation represents a key component of the coagulation process for preventing excessive hemorrhage. The structural characteristics of blood clots are associated with their strength and susceptibility to fibrinolysis. Scanning electron microscopy is a technique that allows for state-of-the-art image capture of blood clots, providing visualization of topography, fibrin thickness, fibrin network density, and blood cell involvement and morphology. In this chapter, we provide a detailed protocol for characterization of plasma and whole blood clot structure using SEM, covering the spectrum from blood collection, in vitro clot formation, sample preparation for SEM, imaging, and image analysis, specifically focusing on the measurement of fibrin fiber thickness.",
keywords = "Clot generation, Fibrin, Plasma, Scanning electron microscope, Whole blood",
author = "Tengyi Cai and Conor McCafferty and \{Van Den Helm\}, Suelyn and Natasha Letunica and Chantal Attard and Paul Monagle and Vera Ignjatovic",
note = "Publisher Copyright: {\textcopyright} 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2023",
doi = "10.1007/978-1-0716-3175-1\_52",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "775--786",
booktitle = "Methods in Molecular Biology",
}