@inbook{cd8e347881454056b82e693c1e9f1aab,
title = "Protocol for detection of hiv-tat protein in cerebrospinal fluid by a sandwich enzyme-linked immunosorbent assay",
abstract = "The human immunodefi ciency virus (HIV) transactivator of transcription (Tat) is a virally produced protein that is required for effi cient viral replication. Once formed inside an infected cell, Tat is secreted into the extracellular space where it has pathophysiological consequences on cells it interacts with. Tat has been demonstrated to be neurotoxic and is produced even under the pressures of anti-retroviral therapy;therefore Tat is suspected to contribute to the development of HIV-associated neurocognitive disorders. In this chapter, we describe a sandwich enzyme-linked immunosorbent assay protocol for the detection of Tat from cerebrospinal fl uid samples.",
keywords = "CSF, Cell lysates, ELISA, HIV, Protein detection, Tat, Transactivator of transcription",
author = "Johnson, {Tory P.} and Avindra Nath",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2016.",
year = "2016",
doi = "10.1007/978-1-4939-3046-3_23",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "343--352",
booktitle = "Methods in Molecular Biology",
}