Protocol for detecting Yersinia pseudotuberculosis nitric oxide exposure during in vitro growth

Rezia Era D. Braza; Katherine L. Cotten; Kimberly M. Davis

doi:10.1016/j.xpro.2022.101760

Protocol for detecting Yersinia pseudotuberculosis nitric oxide exposure during in vitro growth

Rezia Era D. Braza, Katherine L. Cotten, Kimberly M. Davis

Bloomberg School of Public Health

Research output: Contribution to journal › Article › peer-review

Abstract

Yersinia pseudotuberculosis (Yptb) is a bacterial pathogen that causes foodborne illness. Defense against the host antimicrobial gas, nitric oxide (NO), by the bacterial NO-detoxifying gene, hmp, promotes Yptb replication in mouse models of infection. Here, we detail the use of fluorescent signals as readouts for NO exposure within individual cells and subsequent detection of heterogeneity within a population, using single-cell imaging and analysis. This protocol quantifies NO exposure in culture, without capturing the full complexity of the host environment. For complete details on the use and execution of this protocol, please refer to Patel et al. (2021).

Original language	English (US)
Article number	101760
Journal	STAR Protocols
Volume	3
Issue number	4
DOIs	https://doi.org/10.1016/j.xpro.2022.101760
State	Published - Dec 16 2022

Keywords

Cell culture
Microbiology
Microscopy

ASJC Scopus subject areas

General Immunology and Microbiology
General Biochemistry, Genetics and Molecular Biology
General Neuroscience

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10.1016/j.xpro.2022.101760

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abstract = "Yersinia pseudotuberculosis (Yptb) is a bacterial pathogen that causes foodborne illness. Defense against the host antimicrobial gas, nitric oxide (NO), by the bacterial NO-detoxifying gene, hmp, promotes Yptb replication in mouse models of infection. Here, we detail the use of fluorescent signals as readouts for NO exposure within individual cells and subsequent detection of heterogeneity within a population, using single-cell imaging and analysis. This protocol quantifies NO exposure in culture, without capturing the full complexity of the host environment. For complete details on the use and execution of this protocol, please refer to Patel et al. (2021).",

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AB - Yersinia pseudotuberculosis (Yptb) is a bacterial pathogen that causes foodborne illness. Defense against the host antimicrobial gas, nitric oxide (NO), by the bacterial NO-detoxifying gene, hmp, promotes Yptb replication in mouse models of infection. Here, we detail the use of fluorescent signals as readouts for NO exposure within individual cells and subsequent detection of heterogeneity within a population, using single-cell imaging and analysis. This protocol quantifies NO exposure in culture, without capturing the full complexity of the host environment. For complete details on the use and execution of this protocol, please refer to Patel et al. (2021).

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KW - Microscopy

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Protocol for detecting Yersinia pseudotuberculosis nitric oxide exposure during in vitro growth

Abstract

Keywords

ASJC Scopus subject areas

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