TY - JOUR
T1 - Proteomics analyses revealed the reduction of carbon- And nitrogen-metabolism and ginsenoside biosynthesis in the red-skin disorder of Panax ginseng
AU - Ma, Rui
AU - Jiang, Rui
AU - Chen, Xuenan
AU - Zhao, Daqing
AU - Li, Tong
AU - Sun, Liwei
N1 - Funding Information:
This work was supported by the Foundation of the National Key R&D Program (No. 2017YFC1702100), the Jilin Provincial Scientific and Technologic Development Projects (No. 20160307027YY and No. 20180201075YY), the Jilin Provincial Education Department Project in 2016 (No. 76), the Jilin Province Industrial Innovation Special Fund Project (No. 2017C043) and the Jilin Science and Technology Leading Talent Project (No. 201731200).
Publisher Copyright:
© 2019 CSIRO.
PY - 2019
Y1 - 2019
N2 - Red-skin disorder (RSD), a non-infectious disorder in Panax ginseng, impairs the quality and yield of ginseng and impedes continuous cropping. Since the mechanism of this disorder is unknown, there are no effective prevention measures for RSD. The proteomic changes in RSD ginseng were analysed in this study by two-dimensional electrophoresis (2-DE) and isobaric tags for relative and absolute quantification (iTRAQ). The differential expression of 137 proteins (60 from 2-DE and 77 from iTRAQ) was identified in RSD ginseng as compared with healthy ginseng. Most changes are related to carbon- and nitrogen- metabolism, redox homeostasis, and stress resistance. We also found that the concentration of metal elements, such as iron (Fe), aluminium (Al), and manganese (Mn), was significantly increased in RSD ginseng. These increased metals would be chelated with phenols to form red spots on the ginseng epidermis. Moreover, RSD disturbed the carbon and nitrogen metabolism and affected the biosynthesis of nutrients (sugar, proteins, amino acids) and active components (ginsenosides), which reduced the survival rate and medicinal value of ginseng. These differences between RSD and healthy ginseng will contribute to the understanding of RSD mechanism.
AB - Red-skin disorder (RSD), a non-infectious disorder in Panax ginseng, impairs the quality and yield of ginseng and impedes continuous cropping. Since the mechanism of this disorder is unknown, there are no effective prevention measures for RSD. The proteomic changes in RSD ginseng were analysed in this study by two-dimensional electrophoresis (2-DE) and isobaric tags for relative and absolute quantification (iTRAQ). The differential expression of 137 proteins (60 from 2-DE and 77 from iTRAQ) was identified in RSD ginseng as compared with healthy ginseng. Most changes are related to carbon- and nitrogen- metabolism, redox homeostasis, and stress resistance. We also found that the concentration of metal elements, such as iron (Fe), aluminium (Al), and manganese (Mn), was significantly increased in RSD ginseng. These increased metals would be chelated with phenols to form red spots on the ginseng epidermis. Moreover, RSD disturbed the carbon and nitrogen metabolism and affected the biosynthesis of nutrients (sugar, proteins, amino acids) and active components (ginsenosides), which reduced the survival rate and medicinal value of ginseng. These differences between RSD and healthy ginseng will contribute to the understanding of RSD mechanism.
KW - Panax ginseng
KW - RSD
KW - ginsenoside biosynthesis
KW - metal stress
KW - proteomics
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U2 - 10.1071/FP18269
DO - 10.1071/FP18269
M3 - Article
C2 - 31581976
AN - SCOPUS:85073027311
SN - 1445-4408
VL - 46
SP - 1123
EP - 1133
JO - Functional Plant Biology
JF - Functional Plant Biology
IS - 12
ER -