Proteomic evidence for roles for nucleolin and poly[ADP-ribosyl] transferase in drug resistance

Zongming Fu, Catherine Fenselau

Research output: Contribution to journalArticlepeer-review

30 Scopus citations


One-hundred twenty-four proteins have been identified in the soluble nuclear protein mixture from MCF-7 human breast cancer cells, of which more than 90% are classically categorized as nuclear proteins. Proteins were also studied from three drug resistant MDF-7 lines, selected previously from the same parent line by exposure to etoposide, to mitoxantrone, or to adriamycin in the presence of verapamil. Both quantitative gel comparisons and stable isotope labeling were used to identify a total of fourteen proteins whose abundances are altered by more than 2-fold in the three resistant lines. Several cytoskeleton proteins, cytokeratin 8, cytokeratin 19, septin 2, and alpha tropomyosin, are decreased in common across the three resistant cell lines. PARP-1 (poly[ADP-ribosyl]transefrase or connexion) is found to be less abundant in all three resistant lines. Nucleolin is more abundant in lines resistant to etoposide and mitoxantrone, while the mitotic checkpoint protein BUB 3 is more abundant in the line resistant to adriamycin/verapamil.

Original languageEnglish (US)
Pages (from-to)1583-1591
Number of pages9
JournalJournal of proteome research
Issue number5
StatePublished - Sep 2005
Externally publishedYes


  • 2-D gel electrophoresis
  • Adriamycin
  • Drug resistance
  • Etoposide
  • Isotope ratios
  • MCF-7 cancer cells
  • Metabolic labeling
  • Mitoxantrone
  • Nuclear proteins

ASJC Scopus subject areas

  • Biochemistry
  • General Chemistry


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