Proteomic analysis of human vitreous humor

Krishna R. Murthy, Renu Goel, Yashwanth Subbannayya, Harrys K C Jacob, Praveen R. Murthy, Srikanth Srinivas Manda, Arun H. Patil, Rakesh Sharma, Nandini A. Sahasrabuddhe, Arun Parashar, Bipin G. Nair, Venkatarangaiah Krishna, T. S Keshava Prasad, Harsha Gowda, Akhilesh Pandey

Research output: Contribution to journalArticlepeer-review

83 Scopus citations


Background: The vitreous humor is a transparent, gelatinous mass whose main constituent is water. It plays an important role in providing metabolic nutrient requirements of the lens, coordinating eye growth and providing support to the retina. It is in close proximity to the retina and reflects many of the changes occurring in this tissue. The biochemical changes occurring in the vitreous could provide a better understanding about the pathophysiological processes that occur in vitreoretinopathy. In this study, we investigated the proteome of normal human vitreous humor using high resolution Fourier transform mass spectrometry. Results: The vitreous humor was subjected to multiple fractionation techniques followed by LC-MS/MS analysis. We identified 1,205 proteins, 682 of which have not been described previously in the vitreous humor. Most proteins were localized to the extracellular space (24%), cytoplasm (20%) or plasma membrane (14%). Classification based on molecular function showed that 27% had catalytic activity, 10% structural activity, 10% binding activity, 4% cell and 4% transporter activity. Categorization for biological processes showed 28% participate in metabolism, 20% in cell communication and 13% in cell growth. The data have been deposited to the ProteomeXchange with identifier PXD000957. Conclusion: This large catalog of vitreous proteins should facilitate biomedical research into pathological conditions of the eye including diabetic retinopathy, retinal detachment and cataract.

Original languageEnglish (US)
Article number29
JournalClinical Proteomics
Issue number1
StatePublished - Jul 14 2014


  • Body fluid proteomics
  • OFFGEL electrophoresis
  • Protein biomarkers
  • Proteome discoverer
  • Retina
  • SCX chromatography
  • Secreted proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Molecular Medicine
  • Clinical Biochemistry


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