Proteome of synaptosome-associated proteins in spinal cord dorsal horn after peripheral nerve injury

Om V Singh, Myron Yaster, Ji Tian Xu, Yun Guan, Xiaowei Guan, Arun M. Dharmarajan, Srinivasa N. Raja, Pamela Leslie Zeitlin, Yuanxiang Tao

Research output: Contribution to journalArticlepeer-review

37 Scopus citations


Peripheral nerve injury may lead to neuroadaptive changes of cellular signals in spinal cord that are thought to contribute to central mechanisms underlying neuropathic pain. Here we used a 2-DE-based proteomic technique to determine the global expression changes of synaptosome-associated proteins in spinal cord dorsal horn after unilateral fifth spinal nerve injury (SNI). The fifth lumbar dorsal horns ipsilateral to SNI or sham surgery were harvested on day 14 post-surgery, and the total soluble and synaptosomal fractions were isolated. The proteins derived from the synaptosomal fraction were resolved by 2-DE. We identified 27 proteins that displayed different expression levels after SNI, including proteins involved in transmission and modulation of noxious information, cellular metabolism, membrane receptor trafficking, oxidative stress, apoptosis, and degeneration. Six of the 27 proteins were chosen randomly and further validated in the synaptosomal fraction by Western blot analysis. Unexpectedly, Western blot analysis showed that only one protein in the total soluble fraction exhibited a significant expression change after SNI. The data indicate that peripheral nerve injury changes not only protein expression but also protein subcellular distribution in dorsal horn cells. These changes might participate in the central mechanism that underlies the maintenance of neuropathic pain.

Original languageEnglish (US)
Pages (from-to)1241-1253
Number of pages13
Issue number5
StatePublished - Mar 2009


  • Peripheral nerve injury
  • Spinal cord dorsal horn
  • Synaptosomal fraction
  • Two-dimensional electrophoresis

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology


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