Proteome of Rhipicephalus sanguineus tick saliva induced by the secretagogues pilocarpine and dopamine

C. J. Oliveira, E. Anatriello, I. K. de Miranda-Santos, I. M. Francischetti, A. Sá-Nunes, B. R. Ferreira, J. M.C. Ribeiro

Research output: Contribution to journalArticlepeer-review

46 Scopus citations


One dimensional gel electrophoresis was used to separate proteins from the saliva of Rhipicephalus sanguineus female ticks fed on rabbits. Gel slices were subjected to tryptic digestion and analyzed by reversed-phase HPLC followed by MS/MS analysis. The data were compared to a database of salivary proteins of the same tick and to the predicted proteins of the host. Saliva was obtained by either pilocarpine or dopamine stimulation of partially fed ticks. Electrophoretic separations of both yielded products that were identified by mass spectrometry, although the pilocarpine-derived sample was of much better quality. The majority of identified proteins were of rabbit origin, indicating the recycling of the host proteins in the tick saliva, including hemoglobin, albumin, haptoglobin, transferring, and a plasma serpin. The few proteins found that were previously associated with parasitism and blood feeding include 2 glycine-rich, cement-like proteins, 2 lipocalins, and a thyropin protease inhibitor. Among other of the 19 tick proteins identified, albeit with undefined roles, were SPARC and cyclophilin A. This catalog provides a resource that can be mined for secreted molecules that play a role in tick-host interactions.

Original languageEnglish (US)
Pages (from-to)469-477
Number of pages9
JournalTicks and Tick-borne Diseases
Issue number6
StatePublished - Dec 2013
Externally publishedYes


  • Dopamine
  • Pilocarpine
  • Proteome
  • Rhipicephalus sanguineus
  • Tick saliva

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Insect Science
  • Infectious Diseases


Dive into the research topics of 'Proteome of Rhipicephalus sanguineus tick saliva induced by the secretagogues pilocarpine and dopamine'. Together they form a unique fingerprint.

Cite this