Proteome of murine jejunal brush border membrane vesicles

Mark Donowitz; Siddharth Singh; Farah F. Salahuddin; Boris M. Hogema; Yueping Chen; Marjan Gucek; Robert N. Cole; Nicholas Zachos; Olga N Kovbasnjuk; Lynne A. Lapierre; Nellie Broere; James Goldenring; Hugo Dejonge; Xuhang Li

doi:10.1021/pr0701761

Proteome of murine jejunal brush border membrane vesicles

Mark Donowitz, Siddharth Singh, Farah F. Salahuddin, Boris M. Hogema, Yueping Chen, Marjan Gucek, Robert N. Cole, Nicholas Zachos, Olga N Kovbasnjuk, Lynne A. Lapierre, Nellie Broere, James Goldenring, Hugo Dejonge, Xuhang Li

School of Medicine

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

The first detailed description of the proteome of the mouse jejunal brush border membrane vesicle is presented here. This was obtained by a combination of purification via divalent (Mg²⁺) cation precipitation starting with isolated cells plus strong cation exchange chromatography LC-MS/MS. Five-hundred seventy proteins were identified including 45 transport proteins. Among the latter, 18 had either not been identified in the intestine in the past or there was a single unconfirmed report of their presence. Validation was accomplished by a combination of immunoblotting and immunofluorescence using mouse jejunum and previously described antibodies. The validated BB proteins were aquaporin 7, Glut 9b, Na⁺I^- symporter (NIS), and non-gastric H ⁺/K⁺-ATPase. This study helps to further define the brush border membrane vesicle, a preparation which has been widely used to identify transport function of the small intestine.

Original language	English (US)
Pages (from-to)	4068-4079
Number of pages	12
Journal	Journal of proteome research
Volume	6
Issue number	10
DOIs	https://doi.org/10.1021/pr0701761
State	Published - Oct 2007

Keywords

Brush border
LC-MS/MS
Murine small intestine
Proteome
Transport protein

ASJC Scopus subject areas

General Chemistry
Biochemistry

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10.1021/pr0701761

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title = "Proteome of murine jejunal brush border membrane vesicles",

abstract = "The first detailed description of the proteome of the mouse jejunal brush border membrane vesicle is presented here. This was obtained by a combination of purification via divalent (Mg2+) cation precipitation starting with isolated cells plus strong cation exchange chromatography LC-MS/MS. Five-hundred seventy proteins were identified including 45 transport proteins. Among the latter, 18 had either not been identified in the intestine in the past or there was a single unconfirmed report of their presence. Validation was accomplished by a combination of immunoblotting and immunofluorescence using mouse jejunum and previously described antibodies. The validated BB proteins were aquaporin 7, Glut 9b, Na+I- symporter (NIS), and non-gastric H +/K+-ATPase. This study helps to further define the brush border membrane vesicle, a preparation which has been widely used to identify transport function of the small intestine.",

keywords = "Brush border, LC-MS/MS, Murine small intestine, Proteome, Transport protein",

author = "Mark Donowitz and Siddharth Singh and Salahuddin, {Farah F.} and Hogema, {Boris M.} and Yueping Chen and Marjan Gucek and Cole, {Robert N.} and Nicholas Zachos and Kovbasnjuk, {Olga N} and Lapierre, {Lynne A.} and Nellie Broere and James Goldenring and Hugo Dejonge and Xuhang Li",

year = "2007",

month = oct,

doi = "10.1021/pr0701761",

language = "English (US)",

volume = "6",

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AU - Donowitz, Mark

AU - Singh, Siddharth

AU - Salahuddin, Farah F.

AU - Hogema, Boris M.

AU - Chen, Yueping

AU - Gucek, Marjan

AU - Cole, Robert N.

AU - Zachos, Nicholas

AU - Kovbasnjuk, Olga N

AU - Lapierre, Lynne A.

AU - Broere, Nellie

AU - Goldenring, James

AU - Dejonge, Hugo

AU - Li, Xuhang

PY - 2007/10

Y1 - 2007/10

N2 - The first detailed description of the proteome of the mouse jejunal brush border membrane vesicle is presented here. This was obtained by a combination of purification via divalent (Mg2+) cation precipitation starting with isolated cells plus strong cation exchange chromatography LC-MS/MS. Five-hundred seventy proteins were identified including 45 transport proteins. Among the latter, 18 had either not been identified in the intestine in the past or there was a single unconfirmed report of their presence. Validation was accomplished by a combination of immunoblotting and immunofluorescence using mouse jejunum and previously described antibodies. The validated BB proteins were aquaporin 7, Glut 9b, Na+I- symporter (NIS), and non-gastric H +/K+-ATPase. This study helps to further define the brush border membrane vesicle, a preparation which has been widely used to identify transport function of the small intestine.

AB - The first detailed description of the proteome of the mouse jejunal brush border membrane vesicle is presented here. This was obtained by a combination of purification via divalent (Mg2+) cation precipitation starting with isolated cells plus strong cation exchange chromatography LC-MS/MS. Five-hundred seventy proteins were identified including 45 transport proteins. Among the latter, 18 had either not been identified in the intestine in the past or there was a single unconfirmed report of their presence. Validation was accomplished by a combination of immunoblotting and immunofluorescence using mouse jejunum and previously described antibodies. The validated BB proteins were aquaporin 7, Glut 9b, Na+I- symporter (NIS), and non-gastric H +/K+-ATPase. This study helps to further define the brush border membrane vesicle, a preparation which has been widely used to identify transport function of the small intestine.

KW - Brush border

KW - LC-MS/MS

KW - Murine small intestine

KW - Proteome

KW - Transport protein

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Proteome of murine jejunal brush border membrane vesicles

Abstract

Keywords

ASJC Scopus subject areas

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