Protein sulfhydration

Bindu D. Paul; Solomon H. Snyder

doi:10.1016/bs.mie.2014.11.021

Protein sulfhydration

Bindu D. Paul, Solomon H. Snyder

School of Medicine

Research output: Chapter in Book/Report/Conference proceeding › Chapter

36 Scopus citations

Abstract

Hydrogen sulfide (H₂S) is one of the gasotransmitters that modulates various biological processes and participates in multiple signaling pathways. H₂S signals by a process termed sulfhydration. Sulfhydration has recently been recognized as a posttranslational modification similar to nitrosylation. Sulfhydration occurs at reactive cysteine residues in proteins and results in the conversion of an -SH group of cysteine to an -SSH or a persulfide group. Sulfhydration is highly prevalent in vivo, and aberrant sulfhydration patterns have been observed under several pathological conditions ranging from heart disease to neurodegenerative diseases such as Parkinson's disease. The biotin switch assay, originally developed to detect nitrosylation, has been modified to detect sulfhydration. In this chapter, we discuss the physiological roles of sulfhydration and the methodologies used to detect this modification.

Original language	English (US)
Title of host publication	Methods in Enzymology
Publisher	Academic Press Inc.
Pages	79-90
Number of pages	12
DOIs	https://doi.org/10.1016/bs.mie.2014.11.021
State	Published - 2015

Publication series

Name	Methods in Enzymology
Volume	555
ISSN (Print)	0076-6879
ISSN (Electronic)	1557-7988

Keywords

Hydrogen sulfide
Maleimide assay
Modified biotin switch
Nitrosylation
Sulfhydration

ASJC Scopus subject areas

Biochemistry
Molecular Biology

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10.1016/bs.mie.2014.11.021

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abstract = "Hydrogen sulfide (H2S) is one of the gasotransmitters that modulates various biological processes and participates in multiple signaling pathways. H2S signals by a process termed sulfhydration. Sulfhydration has recently been recognized as a posttranslational modification similar to nitrosylation. Sulfhydration occurs at reactive cysteine residues in proteins and results in the conversion of an -SH group of cysteine to an -SSH or a persulfide group. Sulfhydration is highly prevalent in vivo, and aberrant sulfhydration patterns have been observed under several pathological conditions ranging from heart disease to neurodegenerative diseases such as Parkinson's disease. The biotin switch assay, originally developed to detect nitrosylation, has been modified to detect sulfhydration. In this chapter, we discuss the physiological roles of sulfhydration and the methodologies used to detect this modification.",

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AU - Snyder, Solomon H.

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AB - Hydrogen sulfide (H2S) is one of the gasotransmitters that modulates various biological processes and participates in multiple signaling pathways. H2S signals by a process termed sulfhydration. Sulfhydration has recently been recognized as a posttranslational modification similar to nitrosylation. Sulfhydration occurs at reactive cysteine residues in proteins and results in the conversion of an -SH group of cysteine to an -SSH or a persulfide group. Sulfhydration is highly prevalent in vivo, and aberrant sulfhydration patterns have been observed under several pathological conditions ranging from heart disease to neurodegenerative diseases such as Parkinson's disease. The biotin switch assay, originally developed to detect nitrosylation, has been modified to detect sulfhydration. In this chapter, we discuss the physiological roles of sulfhydration and the methodologies used to detect this modification.

KW - Hydrogen sulfide

KW - Maleimide assay

KW - Modified biotin switch

KW - Nitrosylation

KW - Sulfhydration

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Protein sulfhydration

Abstract

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Keywords

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