Protein chip fabrication by capture of nascent polypeptides

Sheng Ce Tao, Heng Zhu

Research output: Contribution to journalArticlepeer-review

82 Scopus citations


The most challenging step in protein microarray fabrication is high-throughput production of proteins. Here we report two similar strategies to fabricate protein chips through capture onto a solid surface of the nascent polypeptides during translation of synthetic or in vitro-transcribed RNAs. Using these approaches, we efficiently fabricated both peptide and protein microarrays at relatively high density. We further demonstrated that such protein chips can be used to analyze protein activity.

Original languageEnglish (US)
Pages (from-to)1253-1254
Number of pages2
JournalNature biotechnology
Issue number10
StatePublished - Oct 2006

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Medicine
  • Biomedical Engineering


Dive into the research topics of 'Protein chip fabrication by capture of nascent polypeptides'. Together they form a unique fingerprint.

Cite this