Protein A - peroxidase: A valuable tool for the localization of antigens

M. Dubois-Dalcq, H. Mcfarland, D. Mcfarlin

Research output: Contribution to journalArticlepeer-review

63 Scopus citations

Abstract

Protein A of Staphylococcus aureus was conjugated to horseradish peroxidase and used in an indirect immunolabeling technique to visualize membrane and viral antigens. The same Protein A-peroxidase conjugate was used with antisera from five different species. Using this indirect test, membrane markers for T and B lymphocytes were labeled with a greater specificity than when peroxidase conjugated anti-immunoglobulin was used in the second step. Viral antigens on cells infected with measles, vesicular stomatitis, herpes or visna virus, respectively, were also stained in the protein A-peroxidase indirect test with a greater specificity than indirect method using anti-immunoglobulin. Paired preparations were examined in the light and electron microscope. Ultrastructural analysis showed that the protein A-peroxidase conjugate penetrated well through fixed viral membranes and resulted in fine resolution of antigenic sites.

Original languageEnglish (US)
Pages (from-to)1201-1206
Number of pages6
JournalJournal of Histochemistry and Cytochemistry
Volume25
Issue number11
StatePublished - 1977
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

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