TY - JOUR
T1 - Protective efficacy of immunization with capsular antigen against experimental infection with bacteroides fragilis
AU - Kasper, Dennis L.
AU - Onderdonk, Andrew B.
AU - Crabb, Joseph
AU - Bartlett, John G.
N1 - Funding Information:
This work was supported by contract no. DAMD-17-78C-8019 from the U.S. Army Medical Research and Development Command.
Funding Information:
Dr. Kasper is the recipient of Research Career Development Award no. IK04 AIOO126 from the National Institute of Allergy and Infectious Diseases.
PY - 1979/12
Y1 - 1979/12
N2 - The protective efficacy afforded by immunization with the capsular antigen of Bacteroides fragilis against abscess formation and bacteremia due to this organism was studied in an experimental rat model of intraabdominal sepsis. Of unimmunized animals, animals immunized with methylated bovine serum albumin and complete Freund’s adjuvant, and animals immunized with lipopolysaccharide of Bacteroides the-taiotaomicron, >90% developed abscesses when challenged intraperitoneally with strains of B. Fragilis or Bacteroides distasonis (given with an enterococcus) or with the cecal contents of meat-fed rats. In contrast, animals immunized with B. fragilis capsular polysaccharide, given with or without methylated bovine serum albumin and complete Freund’s adjuvant, and animals immunized with the outer membrane of B. fragilis strain 23745 were protected to a significant degree from abscesses caused by challenge with B. fragilis or B. distasonis. Such immunization had no overall effect on the development of abscesses in animals challenged with the entire cecal contents of meat-fed rats; however, B. fragilis was eliminated from the abscesses of these animals. Animals immunized with the capsular polysaccharide were protected from early B. fragilis bacteremia.
AB - The protective efficacy afforded by immunization with the capsular antigen of Bacteroides fragilis against abscess formation and bacteremia due to this organism was studied in an experimental rat model of intraabdominal sepsis. Of unimmunized animals, animals immunized with methylated bovine serum albumin and complete Freund’s adjuvant, and animals immunized with lipopolysaccharide of Bacteroides the-taiotaomicron, >90% developed abscesses when challenged intraperitoneally with strains of B. Fragilis or Bacteroides distasonis (given with an enterococcus) or with the cecal contents of meat-fed rats. In contrast, animals immunized with B. fragilis capsular polysaccharide, given with or without methylated bovine serum albumin and complete Freund’s adjuvant, and animals immunized with the outer membrane of B. fragilis strain 23745 were protected to a significant degree from abscesses caused by challenge with B. fragilis or B. distasonis. Such immunization had no overall effect on the development of abscesses in animals challenged with the entire cecal contents of meat-fed rats; however, B. fragilis was eliminated from the abscesses of these animals. Animals immunized with the capsular polysaccharide were protected from early B. fragilis bacteremia.
UR - http://www.scopus.com/inward/record.url?scp=0018688946&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0018688946&partnerID=8YFLogxK
U2 - 10.1093/infdis/140.5.724
DO - 10.1093/infdis/140.5.724
M3 - Article
C2 - 528789
AN - SCOPUS:0018688946
SN - 0022-1899
VL - 140
SP - 724
EP - 731
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 5
ER -