Protection of chimpanzees from high-dose heterologous HIV-1 challenge by DNA vaccination

Jean D. Boyer; Kenneth E. Ugen; Bin Wang; Michael Agadjanyan; Lori Gilbert; Mark L. Bagarazzi; Michael Chattergoon; Patrice Frost; Ali Javadian; Williams V. Williams; Yosef Refaeli; Richard B. Ciccarelli; Daniel Mccallus; Leslie Coney; David B. Weiner

doi:10.1038/nm0597-526

Protection of chimpanzees from high-dose heterologous HIV-1 challenge by DNA vaccination

Jean D. Boyer, Kenneth E. Ugen, Bin Wang, Michael Agadjanyan, Lori Gilbert, Mark L. Bagarazzi, Michael Chattergoon, Patrice Frost, Ali Javadian, Williams V. Williams, Yosef Refaeli, Richard B. Ciccarelli, Daniel Mccallus, Leslie Coney, David B. Weiner

Research output: Contribution to journal › Article › peer-review

319 Scopus citations

Abstract

Novel approaches for the generation of more effective vaccines for HIV-1 are of significant importance. In this report we analyze the immunogenicity and efficacy of an HIV-1 DNA vaccine encoding env, rev and gag/pol in a chimpanzee model system. The immunized animals developed specific cellular and humoral immune responses. Animals were challenged with a heterologous chimpanzee titered stock of HIV-1 SF2 virus and followed for 48 weeks after challenge. Polymerase chain reaction coupled with reverse transcription (RT-PCR) results indicated infection in the control animal, whereas those animals vaccinated with the DNA constructs were protected from the establishment of infection. These studies serve as an important benchmark for the use of DNA vaccine technology for the production of protective immune responses.

Original language	English (US)
Pages (from-to)	526-532
Number of pages	7
Journal	Nature medicine
Volume	3
Issue number	5
DOIs	https://doi.org/10.1038/nm0597-526
State	Published - 1997
Externally published	Yes

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

Access to Document

10.1038/nm0597-526

Cite this

Boyer, J. D., Ugen, K. E., Wang, B., Agadjanyan, M., Gilbert, L., Bagarazzi, M. L., Chattergoon, M., Frost, P., Javadian, A., Williams, W. V., Refaeli, Y., Ciccarelli, R. B., Mccallus, D., Coney, L., & Weiner, D. B. (1997). Protection of chimpanzees from high-dose heterologous HIV-1 challenge by DNA vaccination. Nature medicine, 3(5), 526-532. https://doi.org/10.1038/nm0597-526

@article{3dc65996da3343b1a27d07f2c76b4b54,

title = "Protection of chimpanzees from high-dose heterologous HIV-1 challenge by DNA vaccination",

abstract = "Novel approaches for the generation of more effective vaccines for HIV-1 are of significant importance. In this report we analyze the immunogenicity and efficacy of an HIV-1 DNA vaccine encoding env, rev and gag/pol in a chimpanzee model system. The immunized animals developed specific cellular and humoral immune responses. Animals were challenged with a heterologous chimpanzee titered stock of HIV-1 SF2 virus and followed for 48 weeks after challenge. Polymerase chain reaction coupled with reverse transcription (RT-PCR) results indicated infection in the control animal, whereas those animals vaccinated with the DNA constructs were protected from the establishment of infection. These studies serve as an important benchmark for the use of DNA vaccine technology for the production of protective immune responses.",

author = "Boyer, {Jean D.} and Ugen, {Kenneth E.} and Bin Wang and Michael Agadjanyan and Lori Gilbert and Bagarazzi, {Mark L.} and Michael Chattergoon and Patrice Frost and Ali Javadian and Williams, {Williams V.} and Yosef Refaeli and Ciccarelli, {Richard B.} and Daniel Mccallus and Leslie Coney and Weiner, {David B.}",

year = "1997",

doi = "10.1038/nm0597-526",

language = "English (US)",

volume = "3",

pages = "526--532",

journal = "Nature medicine",

issn = "1078-8956",

publisher = "Nature Publishing Group",

number = "5",

}

TY - JOUR

T1 - Protection of chimpanzees from high-dose heterologous HIV-1 challenge by DNA vaccination

AU - Boyer, Jean D.

AU - Ugen, Kenneth E.

AU - Wang, Bin

AU - Agadjanyan, Michael

AU - Gilbert, Lori

AU - Bagarazzi, Mark L.

AU - Chattergoon, Michael

AU - Frost, Patrice

AU - Javadian, Ali

AU - Williams, Williams V.

AU - Refaeli, Yosef

AU - Ciccarelli, Richard B.

AU - Mccallus, Daniel

AU - Coney, Leslie

AU - Weiner, David B.

PY - 1997

Y1 - 1997

N2 - Novel approaches for the generation of more effective vaccines for HIV-1 are of significant importance. In this report we analyze the immunogenicity and efficacy of an HIV-1 DNA vaccine encoding env, rev and gag/pol in a chimpanzee model system. The immunized animals developed specific cellular and humoral immune responses. Animals were challenged with a heterologous chimpanzee titered stock of HIV-1 SF2 virus and followed for 48 weeks after challenge. Polymerase chain reaction coupled with reverse transcription (RT-PCR) results indicated infection in the control animal, whereas those animals vaccinated with the DNA constructs were protected from the establishment of infection. These studies serve as an important benchmark for the use of DNA vaccine technology for the production of protective immune responses.

AB - Novel approaches for the generation of more effective vaccines for HIV-1 are of significant importance. In this report we analyze the immunogenicity and efficacy of an HIV-1 DNA vaccine encoding env, rev and gag/pol in a chimpanzee model system. The immunized animals developed specific cellular and humoral immune responses. Animals were challenged with a heterologous chimpanzee titered stock of HIV-1 SF2 virus and followed for 48 weeks after challenge. Polymerase chain reaction coupled with reverse transcription (RT-PCR) results indicated infection in the control animal, whereas those animals vaccinated with the DNA constructs were protected from the establishment of infection. These studies serve as an important benchmark for the use of DNA vaccine technology for the production of protective immune responses.

UR - http://www.scopus.com/inward/record.url?scp=16944363710&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=16944363710&partnerID=8YFLogxK

U2 - 10.1038/nm0597-526

DO - 10.1038/nm0597-526

M3 - Article

C2 - 9142121

AN - SCOPUS:16944363710

SN - 1078-8956

VL - 3

SP - 526

EP - 532

JO - Nature medicine

JF - Nature medicine

IS - 5

ER -

Protection of chimpanzees from high-dose heterologous HIV-1 challenge by DNA vaccination

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this