TY - JOUR
T1 - Prospective molecular profiling of melanoma metastases suggests classifiers of immune responsiveness
AU - Wang, Ena
AU - Miller, Lance D.
AU - Ohnmacht, Galen A.
AU - Mocellin, Simone
AU - Perez-Diez, Ainhoa
AU - Petersen, David
AU - Zhao, Yingdong
AU - Simon, Richard
AU - Powell, John I.
AU - Asaki, Esther
AU - Alexander, H. Richard
AU - Duray, Paul H.
AU - Herlyn, Meenhard
AU - Restifo, Nicholas P.
AU - Liu, Edison T.
AU - Rosenberg, Steven A.
AU - Marincola, Francesco M.
PY - 2002/7/1
Y1 - 2002/7/1
N2 - We amplified RNAs from 63 fine needle aspiration (FNA) samples from 37 s.c. melanoma metastases from 25 patients undergoing immunotherapy for hybridization to a 6108-gene human cDNA chip. By prospectively following the history of the lesions, we could correlate transcript patterns with clinical outcome. Cluster analysis revealed a tight relationship among autologous synchronously sampled tumors compared with unrelated lesions (average Pearson's r = 0.83 and 0.7, respectively, P < 0.0003). As reported previously, two subgroups of metastatic melanoma lesions were identified that, however, had no predictive correlation with clinical outcome. Ranking of gene expression data from pretreatment samples identified ∼30 genes predictive of clinical response (P < 0.001). Analysis of their annotations denoted that approximately half of them were related to T-cell regulation, suggesting that immune responsiveness might be predetermined by a tumor microenvironment conducive to immune recognition.
AB - We amplified RNAs from 63 fine needle aspiration (FNA) samples from 37 s.c. melanoma metastases from 25 patients undergoing immunotherapy for hybridization to a 6108-gene human cDNA chip. By prospectively following the history of the lesions, we could correlate transcript patterns with clinical outcome. Cluster analysis revealed a tight relationship among autologous synchronously sampled tumors compared with unrelated lesions (average Pearson's r = 0.83 and 0.7, respectively, P < 0.0003). As reported previously, two subgroups of metastatic melanoma lesions were identified that, however, had no predictive correlation with clinical outcome. Ranking of gene expression data from pretreatment samples identified ∼30 genes predictive of clinical response (P < 0.001). Analysis of their annotations denoted that approximately half of them were related to T-cell regulation, suggesting that immune responsiveness might be predetermined by a tumor microenvironment conducive to immune recognition.
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M3 - Article
C2 - 12097256
AN - SCOPUS:0036645090
SN - 0008-5472
VL - 62
SP - 3581
EP - 3586
JO - Cancer Research
JF - Cancer Research
IS - 13
ER -