Projection structure and molecular architecture of OxlT, a bacterial membrane transporter

Jürgen A.W. Heymann; Rafiquel Sarker; Teruhisa Hirai; Dan Shi; Jacqueline L.S. Milne; Peter C. Maloney; Sriram Subramaniam

doi:10.1093/emboj/20.16.4408

Projection structure and molecular architecture of OxlT, a bacterial membrane transporter

Jürgen A.W. Heymann, Rafiquel Sarker, Teruhisa Hirai, Dan Shi, Jacqueline L.S. Milne, Peter C. Maloney, Sriram Subramaniam

School of Medicine

Research output: Contribution to journal › Article › peer-review

55 Scopus citations

Abstract

The major facilitator superfamily (MFS) represents the largest collection of evolutionarily related members within the class of membrane 'carrier' proteins. OxlT, a representative example of the MFS, is an oxalate-transporting membrane protein in Oxalobacter formigenes. From an electron crystallographic analysis of two-dimensional crystals of OxlT, we have determined the projection structure of this membrane transporter. The projection map at 6 Å resolution indicates the presence of 12 transmembrane helices in each monomer of OxlT, with one set of six helices related to the other set by an approximate internal two-fold axis. The projection map reveals the existence of a central cavity, which we propose to be part of the pathway of oxalate transport. By combining information from the projection map with related biochemical data, we present probable models for the architectural arrangement of transmembrane helices in this protein superfamily.

Original language	English (US)
Pages (from-to)	4408-4413
Number of pages	6
Journal	EMBO Journal
Volume	20
Issue number	16
DOIs	https://doi.org/10.1093/emboj/20.16.4408
State	Published - Aug 15 2001

Keywords

Electron cryo-microscopy
Major facilitator superfamily
Membrane transporter
Two-dimensional crystallization

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Biochemistry, Genetics and Molecular Biology(all)
Immunology and Microbiology(all)

Access to Document

10.1093/emboj/20.16.4408

Cite this

@article{0b0ed5ef4d3443608bdcab98c2519cc4,

title = "Projection structure and molecular architecture of OxlT, a bacterial membrane transporter",

abstract = "The major facilitator superfamily (MFS) represents the largest collection of evolutionarily related members within the class of membrane 'carrier' proteins. OxlT, a representative example of the MFS, is an oxalate-transporting membrane protein in Oxalobacter formigenes. From an electron crystallographic analysis of two-dimensional crystals of OxlT, we have determined the projection structure of this membrane transporter. The projection map at 6 {\AA} resolution indicates the presence of 12 transmembrane helices in each monomer of OxlT, with one set of six helices related to the other set by an approximate internal two-fold axis. The projection map reveals the existence of a central cavity, which we propose to be part of the pathway of oxalate transport. By combining information from the projection map with related biochemical data, we present probable models for the architectural arrangement of transmembrane helices in this protein superfamily.",

keywords = "Electron cryo-microscopy, Major facilitator superfamily, Membrane transporter, Two-dimensional crystallization",

author = "Heymann, {J{\"u}rgen A.W.} and Rafiquel Sarker and Teruhisa Hirai and Dan Shi and Milne, {Jacqueline L.S.} and Maloney, {Peter C.} and Sriram Subramaniam",

year = "2001",

month = aug,

day = "15",

doi = "10.1093/emboj/20.16.4408",

language = "English (US)",

volume = "20",

pages = "4408--4413",

journal = "EMBO Journal",

issn = "0261-4189",

publisher = "Nature Publishing Group",

number = "16",

}

TY - JOUR

T1 - Projection structure and molecular architecture of OxlT, a bacterial membrane transporter

AU - Heymann, Jürgen A.W.

AU - Sarker, Rafiquel

AU - Hirai, Teruhisa

AU - Shi, Dan

AU - Milne, Jacqueline L.S.

AU - Maloney, Peter C.

AU - Subramaniam, Sriram

PY - 2001/8/15

Y1 - 2001/8/15

N2 - The major facilitator superfamily (MFS) represents the largest collection of evolutionarily related members within the class of membrane 'carrier' proteins. OxlT, a representative example of the MFS, is an oxalate-transporting membrane protein in Oxalobacter formigenes. From an electron crystallographic analysis of two-dimensional crystals of OxlT, we have determined the projection structure of this membrane transporter. The projection map at 6 Å resolution indicates the presence of 12 transmembrane helices in each monomer of OxlT, with one set of six helices related to the other set by an approximate internal two-fold axis. The projection map reveals the existence of a central cavity, which we propose to be part of the pathway of oxalate transport. By combining information from the projection map with related biochemical data, we present probable models for the architectural arrangement of transmembrane helices in this protein superfamily.

AB - The major facilitator superfamily (MFS) represents the largest collection of evolutionarily related members within the class of membrane 'carrier' proteins. OxlT, a representative example of the MFS, is an oxalate-transporting membrane protein in Oxalobacter formigenes. From an electron crystallographic analysis of two-dimensional crystals of OxlT, we have determined the projection structure of this membrane transporter. The projection map at 6 Å resolution indicates the presence of 12 transmembrane helices in each monomer of OxlT, with one set of six helices related to the other set by an approximate internal two-fold axis. The projection map reveals the existence of a central cavity, which we propose to be part of the pathway of oxalate transport. By combining information from the projection map with related biochemical data, we present probable models for the architectural arrangement of transmembrane helices in this protein superfamily.

KW - Electron cryo-microscopy

KW - Major facilitator superfamily

KW - Membrane transporter

KW - Two-dimensional crystallization

UR - http://www.scopus.com/inward/record.url?scp=0035881496&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035881496&partnerID=8YFLogxK

U2 - 10.1093/emboj/20.16.4408

DO - 10.1093/emboj/20.16.4408

M3 - Article

C2 - 11500368

AN - SCOPUS:0035881496

SN - 0261-4189

VL - 20

SP - 4408

EP - 4413

JO - EMBO Journal

JF - EMBO Journal

IS - 16

ER -

Projection structure and molecular architecture of OxlT, a bacterial membrane transporter

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this