Progression of myocardial necrosis during reperfusion of ischemic myocardium

Kaname Matsumura, Richmond W. Jeremy, Jutta Schaper, Lewis C. Becker

Research output: Contribution to journalArticlepeer-review

197 Scopus citations


Background - The occurrence of myocyte necrosis during reperfusion of ischemic myocardium is controversial. This study measured myocardial 2- deoxyglucose uptake, correlated with histology, to determine whether loss of viability occurred during reperfusion of ischemic myocardium. Methods and Results - In 12 anesthetized dogs, the left anterior descending coronary artery was occluded for 90 minutes before 4 hours reperfusion. Myocardial blood flow was measured by microspheres and the tracers 14C-2-deoxyglucose and 18F-2-deoxyglucose were injected intravenously after 5 and 180 minutes of reperfusion, respectively. After 240 minutes, the heart was stained with thioflavin-S (size of no-reflow zone) and triphenyl-tetrazolium chloride (TTC, extent of necrosis). Samples from normal, salvaged, and necrotic myocardium were counted for 14C- and 18F-2-deoxyglucose and microspheres. With the use of a three-compartment model of 2-deoxyglucose uptake, the rate constant k3 for phosphorylation of 14C- and 18F-2-deoxyglucose was calculated for each sample. Viability was defined as k3 ≤ 0.125 min-1 (predictive accuracy 88% versus electron microscopy and 97% versus TTC). Among 58 samples from no-reflow regions, 97% were nonviable after 5 minutes of reperfusion (k3=0.096 ± 0.027 min-1). Among 164 samples from salvaged myocardium, 95% were viable after both 5 and 180 minutes of reperfusion (k3=0.170 ± 0.056 min-1 P<.01 versus no-reflow). Among 179 samples from infarcted myocardium, mean k3 after 5 minutes of reperfusion was 0.184 ± 0.070 min-1 and 65% of samples were viable, but after 180 minutes of reperfusion mean k3 had decreased to 0.077 ± 0.032 min-1 (P<.0001) and 98% of samples were nonviable. Conclusions - A large proportion of samples from infarcted myocardium are viable at the end of the ischemic period but lose viability during the first hours of reperfusion.

Original languageEnglish (US)
Pages (from-to)795-804
Number of pages10
Issue number8
StatePublished - Mar 3 1998


  • Ischemia
  • Metabolism
  • Myocardial infarction
  • Radioisotopes
  • Reperfusion

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)


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