TY - JOUR
T1 - Producing nature's gene-chips
T2 - The generation of peptides for display by MHC class I molecules
AU - Shastri, Nilabh
AU - Schwab, Susan
AU - Serwold, Thomas
PY - 2002
Y1 - 2002
N2 - Gene-chips contain thousands of nucleotide sequences that allow simultaneous analysis of the complex mixture of RNAs transcribed in cells. Like these gene-chips, major histocompatibility complex (MHC) class I molecules display a large array of peptides on the cell surface for probing by the CD8+ T cell repertoire. The peptide mixture represents fragments of most, if not all, intracellular proteins. The antigen processing machinery accomplishes the daunting task of sampling these proteins and cleaving them into the precise set of peptides displayed by MHC I molecules. It has long been believed that antigenic peptides arose as by-products of normal protein turnover. Recent evidence, however, suggests that the primary source of peptides is newly synthesized proteins that arise from conventional as well as cryptic translational reading frames. It is increasingly clear that for many peptides the C-terminus is generated in the cytoplasm, and N-terminal trimming occurs in the endoplasmic reticulum in an MHC I - dependent manner. Nature's gene-chips are thus both parsimonious and elegant.
AB - Gene-chips contain thousands of nucleotide sequences that allow simultaneous analysis of the complex mixture of RNAs transcribed in cells. Like these gene-chips, major histocompatibility complex (MHC) class I molecules display a large array of peptides on the cell surface for probing by the CD8+ T cell repertoire. The peptide mixture represents fragments of most, if not all, intracellular proteins. The antigen processing machinery accomplishes the daunting task of sampling these proteins and cleaving them into the precise set of peptides displayed by MHC I molecules. It has long been believed that antigenic peptides arose as by-products of normal protein turnover. Recent evidence, however, suggests that the primary source of peptides is newly synthesized proteins that arise from conventional as well as cryptic translational reading frames. It is increasingly clear that for many peptides the C-terminus is generated in the cytoplasm, and N-terminal trimming occurs in the endoplasmic reticulum in an MHC I - dependent manner. Nature's gene-chips are thus both parsimonious and elegant.
KW - Antigen presentation
KW - Antigen processing
KW - ER proteolysis
KW - Proteasome
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U2 - 10.1146/annurev.immunol.20.100301.064819
DO - 10.1146/annurev.immunol.20.100301.064819
M3 - Review article
C2 - 11861610
AN - SCOPUS:0036218697
SN - 0732-0582
VL - 20
SP - 463
EP - 493
JO - Annual review of immunology
JF - Annual review of immunology
ER -