TY - JOUR
T1 - Procoagulant Activity of Human Neuroblastoma Cell Lines, in Relation to Cell Growth, Differentiation and Cytogenetic Abnormalities
AU - Esumi, Noriko
AU - Imashuku, Shinsaku
AU - Tsunamoto, Kentaro
AU - Todo, Shinjiro
AU - Misawa, Shinichi
AU - Goto, Toshihiro
AU - Fujisawa, Yoshio
PY - 1989/5
Y1 - 1989/5
N2 - Procoagulant activity (PCA) was investigated in relation to cell growth, differentiation, and cytogenetics in seven human neuroblastoma cell lines. Before 5‐bromodeoxyuridine (BrdUrd) treatment, PCA was notably heterogeneous, with the highest activity in NCG (S‐type in morphology) 40‐to 100‐fold greater than the lowest activity in SK‐N‐D2 (N‐type). PCA was not related to 1p abnormalities. After BrdUrd treatment at 5 μg/ml for 6 days, PCA increased 6.8‐fold in GOTO and 2.7‐fold in SK‐N‐DZ with associated growth inhibition and morphological changes (I‐type morphology converted to S‐type in GOTO and N‐type converted to an advanced N‐type in SK‐N‐DZ). In contrast, only growth suppression was observed in 2 other cell lines, and no changes in PCA, growth or morphology were induced in the remaining 3 cell lines.
AB - Procoagulant activity (PCA) was investigated in relation to cell growth, differentiation, and cytogenetics in seven human neuroblastoma cell lines. Before 5‐bromodeoxyuridine (BrdUrd) treatment, PCA was notably heterogeneous, with the highest activity in NCG (S‐type in morphology) 40‐to 100‐fold greater than the lowest activity in SK‐N‐D2 (N‐type). PCA was not related to 1p abnormalities. After BrdUrd treatment at 5 μg/ml for 6 days, PCA increased 6.8‐fold in GOTO and 2.7‐fold in SK‐N‐DZ with associated growth inhibition and morphological changes (I‐type morphology converted to S‐type in GOTO and N‐type converted to an advanced N‐type in SK‐N‐DZ). In contrast, only growth suppression was observed in 2 other cell lines, and no changes in PCA, growth or morphology were induced in the remaining 3 cell lines.
KW - 5‐Bromodeoxyuridine
KW - Chromosome abnormality
KW - Neuroblastoma
KW - Procoagulant activity
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U2 - 10.1111/j.1349-7006.1989.tb02333.x
DO - 10.1111/j.1349-7006.1989.tb02333.x
M3 - Article
C2 - 2502520
AN - SCOPUS:0024820017
SN - 0910-5050
VL - 80
SP - 438
EP - 443
JO - Japanese Journal of Cancer Research
JF - Japanese Journal of Cancer Research
IS - 5
ER -