Probing the structure of RecA-DNA filaments. Advantages of a fluorescent guanine analog

Scott F. Singleton; Alberto I. Roca; Andrew M. Lee; Jie Xiao

doi:10.1016/j.tet.2006.10.092

Probing the structure of RecA-DNA filaments. Advantages of a fluorescent guanine analog

Scott F. Singleton, Alberto I. Roca, Andrew M. Lee, Jie Xiao

School of Medicine

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

The RecA protein of Escherichia coli plays a crucial role in DNA recombination and repair, as well as various aspects of bacterial pathogenicity. The formation of a RecA-ATP-ssDNA complex initiates all RecA activities and yet a complete structural and mechanistic description of this filament has remained elusive. An analysis of RecA-DNA interactions was performed using fluorescently labeled oligonucleotides. A direct comparison was made between fluorescein and several fluorescent nucleosides. The fluorescent guanine analog 6-methylisoxanthopterin (6MI) demonstrated significant advantages over the other fluorophores and represents an important new tool for characterizing RecA-DNA interactions.

Original language	English (US)
Pages (from-to)	3553-3566
Number of pages	14
Journal	Tetrahedron
Volume	63
Issue number	17
DOIs	https://doi.org/10.1016/j.tet.2006.10.092
State	Published - Apr 23 2007

Keywords

DNA repair
Fluorescence
Pteridine nucleoside
Recombination

ASJC Scopus subject areas

Biochemistry
Drug Discovery
Organic Chemistry

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10.1016/j.tet.2006.10.092

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title = "Probing the structure of RecA-DNA filaments. Advantages of a fluorescent guanine analog",

abstract = "The RecA protein of Escherichia coli plays a crucial role in DNA recombination and repair, as well as various aspects of bacterial pathogenicity. The formation of a RecA-ATP-ssDNA complex initiates all RecA activities and yet a complete structural and mechanistic description of this filament has remained elusive. An analysis of RecA-DNA interactions was performed using fluorescently labeled oligonucleotides. A direct comparison was made between fluorescein and several fluorescent nucleosides. The fluorescent guanine analog 6-methylisoxanthopterin (6MI) demonstrated significant advantages over the other fluorophores and represents an important new tool for characterizing RecA-DNA interactions.",

keywords = "DNA repair, Fluorescence, Pteridine nucleoside, Recombination",

author = "Singleton, {Scott F.} and Roca, {Alberto I.} and Lee, {Andrew M.} and Jie Xiao",

note = "Funding Information: This work was supported by a grant to S.F.S. from the NIH (GM58114), and an NSF Minority Postdoctoral Research Fellowship to A.I.R. The authors are grateful to Mary Hawkins (National Cancer Institute, NIH) for provocative discussions and for providing a sample of oligonucleotide 30-6MI used for preliminary investigations. We further thank Dr. Jay Knutson (National Heart, Lung and Blood Institute, NIH) and Dr. Jeff Nichols (Rice University) for spectrofluorometry assistance, and Dr. Steen Pedersen (Baylor College of Medicine) for helpful discussions.",

year = "2007",

month = apr,

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doi = "10.1016/j.tet.2006.10.092",

language = "English (US)",

volume = "63",

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AU - Singleton, Scott F.

AU - Roca, Alberto I.

AU - Lee, Andrew M.

AU - Xiao, Jie

N1 - Funding Information: This work was supported by a grant to S.F.S. from the NIH (GM58114), and an NSF Minority Postdoctoral Research Fellowship to A.I.R. The authors are grateful to Mary Hawkins (National Cancer Institute, NIH) for provocative discussions and for providing a sample of oligonucleotide 30-6MI used for preliminary investigations. We further thank Dr. Jay Knutson (National Heart, Lung and Blood Institute, NIH) and Dr. Jeff Nichols (Rice University) for spectrofluorometry assistance, and Dr. Steen Pedersen (Baylor College of Medicine) for helpful discussions.

PY - 2007/4/23

Y1 - 2007/4/23

N2 - The RecA protein of Escherichia coli plays a crucial role in DNA recombination and repair, as well as various aspects of bacterial pathogenicity. The formation of a RecA-ATP-ssDNA complex initiates all RecA activities and yet a complete structural and mechanistic description of this filament has remained elusive. An analysis of RecA-DNA interactions was performed using fluorescently labeled oligonucleotides. A direct comparison was made between fluorescein and several fluorescent nucleosides. The fluorescent guanine analog 6-methylisoxanthopterin (6MI) demonstrated significant advantages over the other fluorophores and represents an important new tool for characterizing RecA-DNA interactions.

AB - The RecA protein of Escherichia coli plays a crucial role in DNA recombination and repair, as well as various aspects of bacterial pathogenicity. The formation of a RecA-ATP-ssDNA complex initiates all RecA activities and yet a complete structural and mechanistic description of this filament has remained elusive. An analysis of RecA-DNA interactions was performed using fluorescently labeled oligonucleotides. A direct comparison was made between fluorescein and several fluorescent nucleosides. The fluorescent guanine analog 6-methylisoxanthopterin (6MI) demonstrated significant advantages over the other fluorophores and represents an important new tool for characterizing RecA-DNA interactions.

KW - DNA repair

KW - Fluorescence

KW - Pteridine nucleoside

KW - Recombination

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AN - SCOPUS:33947192465

SN - 0040-4020

VL - 63

SP - 3553

EP - 3566

JO - Tetrahedron

JF - Tetrahedron

IS - 17

ER -

Probing the structure of RecA-DNA filaments. Advantages of a fluorescent guanine analog

Abstract

Keywords

ASJC Scopus subject areas

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