TY - JOUR
T1 - Primary structure and functional expression of a cGMP-gated potassium channel
AU - Yao, Xiaoqiang
AU - Segal, Alan S.
AU - Welling, Paul
AU - Zhang, Xiaoqin
AU - Mcnicholas, Carmel M.
AU - Engel, David
AU - Boulpaep, Emile L.
AU - Desir, Gary V.
PY - 1995/12/5
Y1 - 1995/12/5
N2 - Cyclic nucleotides modulate potassium (K) channel activity in many cells and are thought to act indirectly by inducing channel protein phosphorylation. Herein we report the isolation from rabbit of a gene encoding a K channel (Kcn1) that is specifically activated by cGMP and not by cAMP. Analysis of the deduced amino acid sequence (725 amino acids) indicates that, in addition to a core region that is highly homologous to Shaker K channels, Kcn1 also contains a cysteine-rich region similar to that of ligand-gated ion channels and a cyclic nucleotide-binding region. Northern blot analysis detects gene expression in kidney, aorta, and brain. Kcn1 represents a class of K channels that may be specifically regulated by cGMP and could play an important role in mediating the effects of substances, such as nitric oxide, that increase intracellular cGMP.
AB - Cyclic nucleotides modulate potassium (K) channel activity in many cells and are thought to act indirectly by inducing channel protein phosphorylation. Herein we report the isolation from rabbit of a gene encoding a K channel (Kcn1) that is specifically activated by cGMP and not by cAMP. Analysis of the deduced amino acid sequence (725 amino acids) indicates that, in addition to a core region that is highly homologous to Shaker K channels, Kcn1 also contains a cysteine-rich region similar to that of ligand-gated ion channels and a cyclic nucleotide-binding region. Northern blot analysis detects gene expression in kidney, aorta, and brain. Kcn1 represents a class of K channels that may be specifically regulated by cGMP and could play an important role in mediating the effects of substances, such as nitric oxide, that increase intracellular cGMP.
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U2 - 10.1073/pnas.92.25.11711
DO - 10.1073/pnas.92.25.11711
M3 - Article
C2 - 8524834
AN - SCOPUS:0029586341
SN - 0027-8424
VL - 92
SP - 11711
EP - 11715
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 25
ER -