TY - JOUR
T1 - Primary cilia control cellular patterning of Meibomian glands during morphogenesis but not lipid composition
AU - Portal, Céline
AU - Lin, Yvonne
AU - Rastogi, Varuni
AU - Peterson, Cornelia
AU - Yiu, Samuel Chi Hung
AU - Foster, James W.
AU - Wilkerson, Amber
AU - Butovich, Igor A.
AU - Iomini, Carlo
N1 - Funding Information:
The authors thank Qing Liu for his involvement during the early stages of this project, Hoku West-Foyle from the Microscope Facility at Johns Hopkins for his technical assistance, the reference histology core facility at Johns Hopkins University for the paraffin sections, and the members of the Wilmer Cornea Group for their helpful input and discussions. This work was supported by grants from the National Eye Institute, National Institute of Health (EY030661 to CI, EY024324 and EY027349 to IB), a core grant to the Wilmer Eye Institute (EY001765), a grant from the Office of Director, NIH (S10RR024550 to S.C. Kuo, Microscopy Facility at Johns Hopkins University); by a grant from the Eisinger Family; by a Wilmer Eye Institute Seed Fund to CI; and by an unrestricted grant from RPB to the Wilmer Eye Institute.
Funding Information:
The authors thank Qing Liu for his involvement during the early stages of this project, Hoku West-Foyle from the Microscope Facility at Johns Hopkins for his technical assistance, the reference histology core facility at Johns Hopkins University for the paraffin sections, and the members of the Wilmer Cornea Group for their helpful input and discussions. This work was supported by grants from the National Eye Institute, National Institute of Health (EY030661 to CI, EY024324 and EY027349 to IB), a core grant to the Wilmer Eye Institute (EY001765), a grant from the Office of Director, NIH (S10RR024550 to S.C. Kuo, Microscopy Facility at Johns Hopkins University); by a grant from the Eisinger Family; by a Wilmer Eye Institute Seed Fund to CI; and by an unrestricted grant from RPB to the Wilmer Eye Institute.
Publisher Copyright:
© 2023, The Author(s).
PY - 2023/12
Y1 - 2023/12
N2 - Meibomian glands (MGs) are modified sebaceous glands producing the tear film’s lipids. Despite their critical role in maintaining clear vision, the mechanisms underlying MG morphogenesis in development and disease remain obscure. Cilia-mediate signals are critical for the development of skin adnexa, including sebaceous glands. Thus, we investigated the role of cilia in MG morphogenesis during development. Most cells were ciliated during early MG development, followed by cilia disassembly during differentiation. In mature glands, ciliated cells were primarily restricted to the basal layer of the proximal gland central duct. Cilia ablation in keratine14-expressing tissue disrupted the accumulation of proliferative cells at the distal tip but did not affect the overall rate of proliferation or apoptosis. Moreover, impaired cellular patterning during elongation resulted in hypertrophy of mature MGs with increased meibum volume without altering its lipid composition. Thus, cilia signaling networks provide a new platform to design therapeutic treatments for MG dysfunction.
AB - Meibomian glands (MGs) are modified sebaceous glands producing the tear film’s lipids. Despite their critical role in maintaining clear vision, the mechanisms underlying MG morphogenesis in development and disease remain obscure. Cilia-mediate signals are critical for the development of skin adnexa, including sebaceous glands. Thus, we investigated the role of cilia in MG morphogenesis during development. Most cells were ciliated during early MG development, followed by cilia disassembly during differentiation. In mature glands, ciliated cells were primarily restricted to the basal layer of the proximal gland central duct. Cilia ablation in keratine14-expressing tissue disrupted the accumulation of proliferative cells at the distal tip but did not affect the overall rate of proliferation or apoptosis. Moreover, impaired cellular patterning during elongation resulted in hypertrophy of mature MGs with increased meibum volume without altering its lipid composition. Thus, cilia signaling networks provide a new platform to design therapeutic treatments for MG dysfunction.
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U2 - 10.1038/s42003-023-04632-5
DO - 10.1038/s42003-023-04632-5
M3 - Article
C2 - 36932132
AN - SCOPUS:85150421344
SN - 2399-3642
VL - 6
JO - Communications biology
JF - Communications biology
IS - 1
M1 - 282
ER -