Prevention of ischemia-reperfusion injury with a synthetic metalloprotein superoxide dismutase mimic, SC52608

E. Gene Deune, Renee Koopman, Mark E. Smith, Song Pyo Hong, Mustafa R. Ozbek, Roger K. Khouri

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


Superoxide dismutase (SOD) scavenges free superoxide radicals generated during reperfusion of ischemic tissue and decreases cellular injury. A synthetic manganese-based metalloprotien superoxide dismutase mimic, SC52608 (Monsanto Co.), was tested in the isolated rabbit rectus femoris muscle flap to determine its effects on ischemia-reperfusion injury. The results of our experiments analyzing 38 isolated rectus femoris muscles in 19 New Zealand White rabbits show that administration of SC52608 at the onset of 4 hours of warm ischemia anti before reperfusion significantly increases the survival of tim muscle from 20.0±4.9 percent (control, HEPES) to 81.5 ± 4.6 percent (SC52608) (p < 0.001). It preserved functional contraction in 8 of 10 muscles; only 1 of 12 control muscles (cotrol, HEPES) had contractions (p = 0.00151). SC52608 decreased the neutrophil density from 4.63 ± 0.6 x 104 cells/mm2 in the control (HEPES) muscle to 2.71 ± 0.6 X 104cells/mm2 in muscles perfused with SC52608 (p = 0.03). The level of malonyldialdehyde decreased from 6.12 ± 0.26 nmol/gm (control, HEPES) to 4.64 ± 0.41 nmol/gm (SC52608) (p = 0.0028). Postopcrative weights of the muscles showed no statistical differenee (p = 0.14) between the controls (16.0 ± 0.9gm) and the SC52608 (18.1 ± 0.7 gm). Our investigation shows that direct intraarterial infusion of syntthetic superoxide dismutase mimic at the onset of ischemia and prior to reperfusion can reduce reperfusion injury in skeletal muscle.

Original languageEnglish (US)
Pages (from-to)711-718
Number of pages8
JournalPlastic and reconstructive surgery
Issue number4
StatePublished - Sep 1996
Externally publishedYes

ASJC Scopus subject areas

  • Surgery


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