Presence of nicotinic acetylcholine receptors in cat carotid body afferent system

Machiko Shirahata; Yumiko Ishizawa; Maria Rudisill; Brian Schofield; Robert S. Fitzgerald

doi:10.1016/S0006-8993(98)01015-4

Presence of nicotinic acetylcholine receptors in cat carotid body afferent system

Machiko Shirahata, Yumiko Ishizawa, Maria Rudisill, Brian Schofield, Robert S. Fitzgerald

Bloomberg School of Public Health

Research output: Contribution to journal › Article › peer-review

65 Scopus citations

Abstract

With immunocytochemical techniques using a monoclonal antibody for α7 subunits of neuronal nicotinic acetylcholine receptors, we have found these subunits to be exclusively expressed in nerve fibers in the carotid body. Double-immunostaining showed that α7 subunit-positive nerve endings enveloped tyrosine hydroxylase-positive glomus cells. Some carotid sinus nerve fibers and tyrosine hydroxylase-positive petrosal ganglion neurons also expressed α7 subunits. These data support a role for acetylcholine in carotid body neurotransmission.

Original language	English (US)
Pages (from-to)	213-217
Number of pages	5
Journal	Brain research
Volume	814
Issue number	1-2
DOIs	https://doi.org/10.1016/S0006-8993(98)01015-4
State	Published - Dec 14 1998

Keywords

Antibody
Chemoreceptor
Immunocytochemistry
Tyrosine hydroxylase
α7 subunit

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/S0006-8993(98)01015-4

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title = "Presence of nicotinic acetylcholine receptors in cat carotid body afferent system",

abstract = "With immunocytochemical techniques using a monoclonal antibody for α7 subunits of neuronal nicotinic acetylcholine receptors, we have found these subunits to be exclusively expressed in nerve fibers in the carotid body. Double-immunostaining showed that α7 subunit-positive nerve endings enveloped tyrosine hydroxylase-positive glomus cells. Some carotid sinus nerve fibers and tyrosine hydroxylase-positive petrosal ganglion neurons also expressed α7 subunits. These data support a role for acetylcholine in carotid body neurotransmission.",

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author = "Machiko Shirahata and Yumiko Ishizawa and Maria Rudisill and Brian Schofield and Fitzgerald, {Robert S.}",

note = "Funding Information: This work was supported by NHLBI HL 50712, HL47044, and NIEHS ES03819.",

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AU - Shirahata, Machiko

AU - Ishizawa, Yumiko

AU - Rudisill, Maria

AU - Schofield, Brian

AU - Fitzgerald, Robert S.

N1 - Funding Information: This work was supported by NHLBI HL 50712, HL47044, and NIEHS ES03819.

PY - 1998/12/14

Y1 - 1998/12/14

N2 - With immunocytochemical techniques using a monoclonal antibody for α7 subunits of neuronal nicotinic acetylcholine receptors, we have found these subunits to be exclusively expressed in nerve fibers in the carotid body. Double-immunostaining showed that α7 subunit-positive nerve endings enveloped tyrosine hydroxylase-positive glomus cells. Some carotid sinus nerve fibers and tyrosine hydroxylase-positive petrosal ganglion neurons also expressed α7 subunits. These data support a role for acetylcholine in carotid body neurotransmission.

AB - With immunocytochemical techniques using a monoclonal antibody for α7 subunits of neuronal nicotinic acetylcholine receptors, we have found these subunits to be exclusively expressed in nerve fibers in the carotid body. Double-immunostaining showed that α7 subunit-positive nerve endings enveloped tyrosine hydroxylase-positive glomus cells. Some carotid sinus nerve fibers and tyrosine hydroxylase-positive petrosal ganglion neurons also expressed α7 subunits. These data support a role for acetylcholine in carotid body neurotransmission.

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KW - Chemoreceptor

KW - Immunocytochemistry

KW - Tyrosine hydroxylase

KW - α7 subunit

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Presence of nicotinic acetylcholine receptors in cat carotid body afferent system

Abstract

Keywords

ASJC Scopus subject areas

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