Preparation and characterization of hydroperoxy-eicosatetraenoic acids (HPETEs)

J. M. Boeynaems; J. A. Oates; W. C. Hubbard

doi:10.1016/0090-6980(80)90156-2

Preparation and characterization of hydroperoxy-eicosatetraenoic acids (HPETEs)

J. M. Boeynaems, J. A. Oates, W. C. Hubbard

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

5-, 8-, 9-, 11-, 12- and 15 hydroperoxy-eicosatetraenoic acids (HPETEs) were generated from arachidonic acid by a reaction with H₂O₂ and Cu++ ions. They were purified by high performance liquid chromatography, either on a silica gel (μ Porasil) column or on a reversed phase (μ Bondapak C₁₈) column. The yield of 5-HPETE was considerably greater when the μ Bondapak C₁₈ column was used. The HPETEs were characterized and assayed by their ability to oxidize triphenylphosphine: triphenylphosphine oxide formation was monitored by gas chromatography. When stored in methylene chloride at -20°C, the HPETEs were stable for several months.

Original language	English (US)
Pages (from-to)	87-97
Number of pages	11
Journal	Prostaglandins
Volume	19
Issue number	1
DOIs	https://doi.org/10.1016/0090-6980(80)90156-2
State	Published - Jan 1980
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Endocrinology

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10.1016/0090-6980(80)90156-2

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abstract = "5-, 8-, 9-, 11-, 12- and 15 hydroperoxy-eicosatetraenoic acids (HPETEs) were generated from arachidonic acid by a reaction with H2O2 and Cu++ ions. They were purified by high performance liquid chromatography, either on a silica gel (μ Porasil) column or on a reversed phase (μ Bondapak C18) column. The yield of 5-HPETE was considerably greater when the μ Bondapak C18 column was used. The HPETEs were characterized and assayed by their ability to oxidize triphenylphosphine: triphenylphosphine oxide formation was monitored by gas chromatography. When stored in methylene chloride at -20°C, the HPETEs were stable for several months.",

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AU - Oates, J. A.

AU - Hubbard, W. C.

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AB - 5-, 8-, 9-, 11-, 12- and 15 hydroperoxy-eicosatetraenoic acids (HPETEs) were generated from arachidonic acid by a reaction with H2O2 and Cu++ ions. They were purified by high performance liquid chromatography, either on a silica gel (μ Porasil) column or on a reversed phase (μ Bondapak C18) column. The yield of 5-HPETE was considerably greater when the μ Bondapak C18 column was used. The HPETEs were characterized and assayed by their ability to oxidize triphenylphosphine: triphenylphosphine oxide formation was monitored by gas chromatography. When stored in methylene chloride at -20°C, the HPETEs were stable for several months.

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Preparation and characterization of hydroperoxy-eicosatetraenoic acids (HPETEs)

Abstract

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