TY - GEN
T1 - Preliminary characterization of a glucose-sensitive hydrogel
AU - Beier, Brooke L.
AU - Brandner, Eric M.
AU - Musick, Katherine M.
AU - Matsumoto, Akira
AU - Panitch, Alyssa
AU - Nauman, Eric A.
AU - Irazoqui, Pedro P.
PY - 2010
Y1 - 2010
N2 - We present proof-of-concept studies that display the potential for using a glucose-sensitive hydrogel as a continuous glucose sensor. A study to characterize the swelling ratio of the hydrogel at normal physiological and pathological hyperglycemic glucose levels was performed. The hydrogel exposed to the hyperglycemic glucose solution had a higher equilibrium swelling ratio than the hydrogel exposed to the normal glucose concentration solution. The diffusivity of a small molecule, fluorescein isothiocyanate (FITC), through a hydrogel exposed to a hyperglycemic solution was determined using fluorescence recovery after photobleaching (FRAP). The diffusivity was found to be 4.2 × 10-14 m2/s, a value approximately four orders of magnitude smaller than the diffusivity of FITC in glucose solution. The permeability of the hydrogel after equilibration in a hyperglycemic solution was found to be 5.1 × 10-17 m2, in the range of 2-4% agarose gels.
AB - We present proof-of-concept studies that display the potential for using a glucose-sensitive hydrogel as a continuous glucose sensor. A study to characterize the swelling ratio of the hydrogel at normal physiological and pathological hyperglycemic glucose levels was performed. The hydrogel exposed to the hyperglycemic glucose solution had a higher equilibrium swelling ratio than the hydrogel exposed to the normal glucose concentration solution. The diffusivity of a small molecule, fluorescein isothiocyanate (FITC), through a hydrogel exposed to a hyperglycemic solution was determined using fluorescence recovery after photobleaching (FRAP). The diffusivity was found to be 4.2 × 10-14 m2/s, a value approximately four orders of magnitude smaller than the diffusivity of FITC in glucose solution. The permeability of the hydrogel after equilibration in a hyperglycemic solution was found to be 5.1 × 10-17 m2, in the range of 2-4% agarose gels.
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U2 - 10.1109/IEMBS.2010.5627210
DO - 10.1109/IEMBS.2010.5627210
M3 - Conference contribution
C2 - 21096685
AN - SCOPUS:78650819011
SN - 9781424441235
T3 - 2010 Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC'10
SP - 5014
EP - 5017
BT - 2010 Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC'10
T2 - 2010 32nd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC'10
Y2 - 31 August 2010 through 4 September 2010
ER -