TY - JOUR
T1 - Pregnancy following transfer of ooplasm from cryopreserved-thawed donor oocytes into recipient oocytes
AU - Lanzendorf, Susan E.
AU - Mayer, Jacob F.
AU - Toner, James
AU - Oehninger, Sergio
AU - Saffan, David S.
AU - Muasher, Suheil
PY - 1999/3
Y1 - 1999/3
N2 - Objective: To determine if frozen-thawed donor oocytes could be used to provide cytoplasm for transfer into patients' oocytes to improve subsequent embryonic development. Design: Prospective evaluation of the procedure in consenting IVF patients. Setting: Assisted reproductive technology program. Patient(s): The study was open to consenting IVF patients (of any age) with a history of poor embryo quality or those couples in which the wife's age was ≥40 years. Intervention(s): Transfer of donor egg cytoplasm from frozen- thawed oocytes into the oocytes of infertile recipients. Main Outcome Measure(s): Donor oocyte survival following cryopreservation, fertilization following cytoplasmic transfer into recipient oocytes, embryo quality, and pregnancy outcome. Result(s): Oocytes collected from four donors were cryopreserved and 61% (28/46) survived the thaw procedure. Cytoplasmic transfer was performed on the eggs of four patients, with fertilization occurring in 70.3% (26/37). Twin pregnancy was established in one patient (35 years of age) with a history of poor embryo quality. Conclusion(s): Cryopreserved donor oocytes may provide a source of cytoplasm for transfer into recipient oocytes, eliminating the need for cycle synchronization between donor and infertile patient.
AB - Objective: To determine if frozen-thawed donor oocytes could be used to provide cytoplasm for transfer into patients' oocytes to improve subsequent embryonic development. Design: Prospective evaluation of the procedure in consenting IVF patients. Setting: Assisted reproductive technology program. Patient(s): The study was open to consenting IVF patients (of any age) with a history of poor embryo quality or those couples in which the wife's age was ≥40 years. Intervention(s): Transfer of donor egg cytoplasm from frozen- thawed oocytes into the oocytes of infertile recipients. Main Outcome Measure(s): Donor oocyte survival following cryopreservation, fertilization following cytoplasmic transfer into recipient oocytes, embryo quality, and pregnancy outcome. Result(s): Oocytes collected from four donors were cryopreserved and 61% (28/46) survived the thaw procedure. Cytoplasmic transfer was performed on the eggs of four patients, with fertilization occurring in 70.3% (26/37). Twin pregnancy was established in one patient (35 years of age) with a history of poor embryo quality. Conclusion(s): Cryopreserved donor oocytes may provide a source of cytoplasm for transfer into recipient oocytes, eliminating the need for cycle synchronization between donor and infertile patient.
KW - Cytoplasmic transfer
KW - In vitro fertilization
KW - Intracytoplasmic sperm injection
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U2 - 10.1016/S0015-0282(98)00504-4
DO - 10.1016/S0015-0282(98)00504-4
M3 - Article
C2 - 10065803
AN - SCOPUS:0032936931
SN - 0015-0282
VL - 71
SP - 575
EP - 577
JO - Fertility and sterility
JF - Fertility and sterility
IS - 3
ER -