@inproceedings{80a844a8637345caab98e6ea3406a06a,
title = "Pre-b{\"o}tzinger complex neurokinin-1 receptor expressing neurons in primary cell culture",
abstract = "Phenotypic identification of pre-B{\"o}tzinger complex (pre-B{\"o}tC) neurons is vital to the delineation of endogenous cellular properties involved in respiratory rhythm generation and neuromodulation of breathing. A subpopulation of pre-B{\"o}tC neurons endowed with intrinsic rhythmicity is proposed as the kernel for inspiratory rhythm generation in vitro. In this study, the pre-B{\"o}tC {"}island{"} preparation was excised from medullary slices and reduced to its heterogeneous cellular constituents using primary cell culture techniques. Cultured neurons were labeled with tetramethylrhodamine conjugated to Substance P (TMR-SP) to identify individual NK-1R expressing neurons. Data from this study provide initial evidence that the pre-B{\"o}tC neurons living in culture remain functionally capable of binding and internalizing the fluorescently-tagged ligand and TMR-SP tagging is a useful experimental tool that reliably identifies the NK-1R phenotype.",
author = "Johnson, {Shere{\'e} M.}",
year = "2008",
doi = "10.1007/978-0-387-73693-8_16",
language = "English (US)",
isbn = "9780387736921",
volume = "605",
series = "Advances in Experimental Medicine and Biology",
pages = "94--98",
booktitle = "Advances in Experimental Medicine and Biology",
}