TY - JOUR
T1 - pp60(c-src) has less affinity for the detergent-insoluble cellular matrix than do pp60(v-src) and other viral protein-tyrosine kinases
AU - Loeb, D. M.
AU - Woolford, J.
AU - Beemon, K.
PY - 1987
Y1 - 1987
N2 - A difference in affinity for a Nonidet P-40-insoluble cellular matrix was observed between the products of the viral and cellular src genes. It has previously been demonstrated that pp60(v-src) is associated with a detergent-insoluble matrix containing the cellular cytoskeleton. (J.G. Burr, G. Dreyfuss, S. Penman, and J.M. Buchanan, Proc. Natl. Acad. Sci. USA 77:3488, 1980). We observed a similar association of the transforming proteins of Fujinami sarcoma virus (P130(gag-fps)) and Yamaguchi 73 avian sarcoma virus (P90(gag-yes)), both of which are tyrosine-specific protein kinases. However, we found that the endogenous c-src product, pp60(c-src), was not tightly bound to the detergent-insoluble matrix. This does not appear to have been due to differences in the cytoskeleton between transformed and nontransformed cells since pp60(c-src) was also solubilized by nonionic detergent in cells transformed by Rous sarcoma virus. This difference in the affinities of the v-src and c-src products for cytoskeletal proteins may contribute to the inability of pp60(c-src) to transform cells.
AB - A difference in affinity for a Nonidet P-40-insoluble cellular matrix was observed between the products of the viral and cellular src genes. It has previously been demonstrated that pp60(v-src) is associated with a detergent-insoluble matrix containing the cellular cytoskeleton. (J.G. Burr, G. Dreyfuss, S. Penman, and J.M. Buchanan, Proc. Natl. Acad. Sci. USA 77:3488, 1980). We observed a similar association of the transforming proteins of Fujinami sarcoma virus (P130(gag-fps)) and Yamaguchi 73 avian sarcoma virus (P90(gag-yes)), both of which are tyrosine-specific protein kinases. However, we found that the endogenous c-src product, pp60(c-src), was not tightly bound to the detergent-insoluble matrix. This does not appear to have been due to differences in the cytoskeleton between transformed and nontransformed cells since pp60(c-src) was also solubilized by nonionic detergent in cells transformed by Rous sarcoma virus. This difference in the affinities of the v-src and c-src products for cytoskeletal proteins may contribute to the inability of pp60(c-src) to transform cells.
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U2 - 10.1128/jvi.61.8.2420-2427.1987
DO - 10.1128/jvi.61.8.2420-2427.1987
M3 - Article
C2 - 2439705
AN - SCOPUS:0023160811
SN - 0022-538X
VL - 61
SP - 2420
EP - 2427
JO - Journal of virology
JF - Journal of virology
IS - 8
ER -