Poteomic analysis of human osteoarthritis synovial fluid

Lavanya Balakrishnan, Raja Sekhar Nirujogi, Sartaj Ahmad, Mitali Bhattacharjee, Srikanth S. Manda, Santosh Renuse, Dhanashree S. Kelkar, Yashwanth Subbannayya, Rajesh Raju, Renu Goel, Joji Kurian Thomas, Navjyot Kaur, Mukesh Dhillon, Shantal Gupta Tankala, Ramesh Jois, Vivek Vasdev, Y. L. Ramachandra, Nandini A. Sahasrabuddhe, T. S Keshava Prasad, Sujatha MohanHarsha Gowda, Subramanian Shankar, Akhilesh Pandey

Research output: Contribution to journalArticlepeer-review

77 Scopus citations


Background: Osteoarthritis is a chronic musculoskeletal disorder characterized mainly by progressive degradation of the hyaline cartilage. Patients with osteoarthritis often postpone seeking medical help, which results in the diagnosis being made at an advanced stage of cartilage destruction. Sustained efforts are needed to identify specific markers that might help in early diagnosis, monitoring disease progression and in improving therapeutic outcomes. We employed a multipronged proteomic approach, which included multiple fractionation strategies followed by high resolution mass spectrometry analysis to explore the proteome of synovial fluid obtained from osteoarthritis patients. In addition to the total proteome, we also enriched glycoproteins from synovial fluid using lectin affinity chromatography. Results: We identified 677 proteins from synovial fluid of patients with osteoarthritis of which 545 proteins have not been previously reported. These novel proteins included ADAM-like decysin 1 (ADAMDEC1), alanyl (membrane) aminopeptidase (ANPEP), CD84, fibulin 1 (FBLN1), matrix remodelling associated 5 (MXRA5), secreted phosphoprotein 2 (SPP2) and spondin 2 (SPON2). We identified 300 proteins using lectin affinity chromatography, including the glycoproteins afamin (AFM), attractin (ATRN), fibrillin 1 (FBN1), transferrin (TF), tissue inhibitor of metalloproteinase 1 (TIMP1) and vasorin (VSN). Gene ontology analysis confirmed that a majority of the identified proteins were extracellular and are mostly involved in cell communication and signaling. We also confirmed the expression of ANPEP, dickkopf WNT signaling pathway inhibitor 3 (DKK3) and osteoglycin (OGN) by multiple reaction monitoring (MRM) analysis of osteoarthritis synovial fluid samples. Conclusions: We present an in-depth analysis of the synovial fluid proteome from patients with osteoarthritis. We believe that the catalog of proteins generated in this study will further enhance our knowledge regarding the pathophysiology of osteoarthritis and should assist in identifying better biomarkers for early diagnosis.

Original languageEnglish (US)
Article number6
JournalClinical Proteomics
Issue number1
StatePublished - 2014


  • Body fluid
  • Cartilage
  • Glycosylation
  • Joint destruction

ASJC Scopus subject areas

  • Molecular Biology
  • Molecular Medicine
  • Clinical Biochemistry


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