Postinjection kinetics of antepartum Rh immune globulin

Frank R. Witter, Rosetta S. Shirey, Suzy L. Nicol, Paul M. Ness

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


The disappearance kinetics of a standard dose of 300 μg of Rh immune globulin given at 28 weeks' gestation was investigated. Ten Rh-negative unsensitized women were given 300 μg of Rh immune globulin at 28 weeks and blood samples were drawn at weekly intervals until delivery. Sera were titrated with Ror red blood cells in a saline solution-antiglobulin method. Titers ranged from 1 to 4 by the first week after injection. In nine of 10 patients the titers were zero at 49 to 70 days after injection. With R2R2 red blood cells and LISS, papain, polybrene, or a combination of methods, Rh immune globulin could still be detected until delivery in four of the nine patients. Because these serologic methods can detect 5 ng/ml anti-D, five of 10 subjects may not have been protected for 8 to 29 days before delivery. This study indicates that a titer of ≤4 is expected for passively acquired anti-D during pregnancy, and that antepartum administration of Rh immune globulin may not always produce detectable passive antibody for as long as theoretically predicted.

Original languageEnglish (US)
Pages (from-to)784-786
Number of pages3
JournalAmerican journal of obstetrics and gynecology
Issue number3
StatePublished - Sep 1990


  • Rh disease
  • Rh hemolytic disease
  • Rh sensitization
  • erythroblastosis fetalis

ASJC Scopus subject areas

  • Obstetrics and Gynecology


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