Positional Dependence of DNA Hole Transfer Efficiency in Nucleosome Core Particles

Huabing Sun; Liwei Zheng; Kun Yang; Marc M. Greenberg

doi:10.1021/jacs.9b03686

Positional Dependence of DNA Hole Transfer Efficiency in Nucleosome Core Particles

Huabing Sun, Liwei Zheng, Kun Yang, Marc M. Greenberg

Research output: Contribution to journal › Article › peer-review

Abstract

Electron deficient "holes" migrate over long distances through the π-system in free DNA. Hole transfer efficiency (HTE) is strongly dependent on sequence and π-stacking. However, there is no consensus regarding the effects of nucleosome core particle (NCP) environment on hole migration. We quantitatively determined HTE in free DNA and NCPs by independently generating holes at specific positions in DNA. The relative HTE varied widely with respect to position within the NCP and proximity to tyrosine, which suppresses hole transfer. These data indicate that hole transfer in chromatin will be affected by the DNA sequence and its position with respect to histone proteins within NCPs.

Original language	English (US)
Pages (from-to)	10154-10158
Number of pages	5
Journal	Journal of the American Chemical Society
Volume	141
Issue number	26
DOIs	https://doi.org/10.1021/jacs.9b03686
State	Published - Jul 3 2019
Externally published	Yes

ASJC Scopus subject areas

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

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10.1021/jacs.9b03686

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abstract = "Electron deficient {"}holes{"} migrate over long distances through the π-system in free DNA. Hole transfer efficiency (HTE) is strongly dependent on sequence and π-stacking. However, there is no consensus regarding the effects of nucleosome core particle (NCP) environment on hole migration. We quantitatively determined HTE in free DNA and NCPs by independently generating holes at specific positions in DNA. The relative HTE varied widely with respect to position within the NCP and proximity to tyrosine, which suppresses hole transfer. These data indicate that hole transfer in chromatin will be affected by the DNA sequence and its position with respect to histone proteins within NCPs.",

author = "Huabing Sun and Liwei Zheng and Kun Yang and Greenberg, {Marc M.}",

note = "Funding Information: We are grateful for generous financial support from the National Institute of General Medical Science (GM-054996). L.Z. thanks Johns Hopkins University for the Glen E. Meyer {\textquoteright}39 Fellowship. Publisher Copyright: {\textcopyright} 2019 American Chemical Society.",

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AU - Sun, Huabing

AU - Zheng, Liwei

AU - Yang, Kun

AU - Greenberg, Marc M.

N1 - Funding Information: We are grateful for generous financial support from the National Institute of General Medical Science (GM-054996). L.Z. thanks Johns Hopkins University for the Glen E. Meyer ’39 Fellowship. Publisher Copyright: © 2019 American Chemical Society.

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N2 - Electron deficient "holes" migrate over long distances through the π-system in free DNA. Hole transfer efficiency (HTE) is strongly dependent on sequence and π-stacking. However, there is no consensus regarding the effects of nucleosome core particle (NCP) environment on hole migration. We quantitatively determined HTE in free DNA and NCPs by independently generating holes at specific positions in DNA. The relative HTE varied widely with respect to position within the NCP and proximity to tyrosine, which suppresses hole transfer. These data indicate that hole transfer in chromatin will be affected by the DNA sequence and its position with respect to histone proteins within NCPs.

AB - Electron deficient "holes" migrate over long distances through the π-system in free DNA. Hole transfer efficiency (HTE) is strongly dependent on sequence and π-stacking. However, there is no consensus regarding the effects of nucleosome core particle (NCP) environment on hole migration. We quantitatively determined HTE in free DNA and NCPs by independently generating holes at specific positions in DNA. The relative HTE varied widely with respect to position within the NCP and proximity to tyrosine, which suppresses hole transfer. These data indicate that hole transfer in chromatin will be affected by the DNA sequence and its position with respect to histone proteins within NCPs.

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Positional Dependence of DNA Hole Transfer Efficiency in Nucleosome Core Particles

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