TY - JOUR
T1 - Position of the kissing-loop interaction associated with PTE-type 3'CITEs can affect enhancement of cap-independent translation
AU - Chattopadhyay, Maitreyi
AU - Kuhlmann, Micki M.
AU - Kumar, Kalyani
AU - Simon, Anne E.
PY - 2014/6
Y1 - 2014/6
N2 - The Panicum mosaic virus-like translation enhancer (PTE) functions as a cap-independent translation enhancer (3'CITE) in members of several Tombusviridae genera including 7/19 carmoviruses. For nearly all PTE, a kissing-loop connects the element with a hairpin found in several conserved locations in the genomic RNA (5' terminal hairpin or ~100. nt from the 5' end) and small subgenomic RNA (~63. nt from the 5' end). Moving the interaction closer to the 5' end in reporter mRNAs using Saguaro cactus virus (SCV) sequences had either a minimal or substantial negative effect on translation. Movement of the kissing loop from position 104 to the SCV 5' terminal hairpin also reduced translation by 4-fold. These results suggest that relocating the PTE kissing loop closer to the 5' end reduces PTE efficiency, in contrast to results for the Barley yellow dwarf BTE and Tomato bushy stunt virus Y-shaped 3'CITEs, suggesting that different 3'CITEs have different bridging requirements.
AB - The Panicum mosaic virus-like translation enhancer (PTE) functions as a cap-independent translation enhancer (3'CITE) in members of several Tombusviridae genera including 7/19 carmoviruses. For nearly all PTE, a kissing-loop connects the element with a hairpin found in several conserved locations in the genomic RNA (5' terminal hairpin or ~100. nt from the 5' end) and small subgenomic RNA (~63. nt from the 5' end). Moving the interaction closer to the 5' end in reporter mRNAs using Saguaro cactus virus (SCV) sequences had either a minimal or substantial negative effect on translation. Movement of the kissing loop from position 104 to the SCV 5' terminal hairpin also reduced translation by 4-fold. These results suggest that relocating the PTE kissing loop closer to the 5' end reduces PTE efficiency, in contrast to results for the Barley yellow dwarf BTE and Tomato bushy stunt virus Y-shaped 3'CITEs, suggesting that different 3'CITEs have different bridging requirements.
KW - Cap-independent translation
KW - Carmovirus
KW - Long-distance RNA:RNA interactions
KW - RNA virus translation
KW - Saguaro cactus virus
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U2 - 10.1016/j.virol.2014.03.027
DO - 10.1016/j.virol.2014.03.027
M3 - Article
C2 - 24928038
AN - SCOPUS:84899835698
SN - 0042-6822
VL - 458-459
SP - 43
EP - 52
JO - Virology
JF - Virology
IS - 1
ER -