POSaM: a fast, flexible, open-source, inkjet oligonucleotide synthesizer and microarrayer

Christopher Lausted; Timothy Dahl; Charles Warren; Kimberly King; Kimberly Smith; Michael Johnson; Ramsey Saleem; John Aitchison; Lee Hood; Stephen R. Lasky

doi:10.1186/gb-2004-5-8-r58

POSaM: a fast, flexible, open-source, inkjet oligonucleotide synthesizer and microarrayer

Christopher Lausted, Timothy Dahl, Charles Warren, Kimberly King, Kimberly Smith, Michael Johnson, Ramsey Saleem, John Aitchison, Lee Hood, Stephen R. Lasky

Research output: Contribution to journal › Article › peer-review

65 Scopus citations

Abstract

DNA arrays are valuable tools in molecular biology laboratories. Their rapid acceptance was aided by the release of plans for a pin-spotting microarrayer by researchers at Stanford. Inkjet microarraying is a flexible, complementary technique that allows the synthesis of arrays of any oligonucleotide sequences de novo. We describe here an open-source inkjet arrayer capable of rapidly producing sets of unique 9,800-feature arrays.

Original language	English (US)
Article number	R58
Journal	Genome biology
Volume	5
Issue number	8
DOIs	https://doi.org/10.1186/gb-2004-5-8-r58
State	Published - Jul 2004
Externally published	Yes

Keywords

Additional Data File
Oligonucleotide Synthesis
Phosphoramidite
Print Head
Propylene Carbonate

ASJC Scopus subject areas

Genetics
Ecology, Evolution, Behavior and Systematics
Cell Biology

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10.1186/gb-2004-5-8-r58

Cite this

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title = "POSaM: a fast, flexible, open-source, inkjet oligonucleotide synthesizer and microarrayer",

abstract = "DNA arrays are valuable tools in molecular biology laboratories. Their rapid acceptance was aided by the release of plans for a pin-spotting microarrayer by researchers at Stanford. Inkjet microarraying is a flexible, complementary technique that allows the synthesis of arrays of any oligonucleotide sequences de novo. We describe here an open-source inkjet arrayer capable of rapidly producing sets of unique 9,800-feature arrays.",

keywords = "Additional Data File, Oligonucleotide Synthesis, Phosphoramidite, Print Head, Propylene Carbonate",

author = "Christopher Lausted and Timothy Dahl and Charles Warren and Kimberly King and Kimberly Smith and Michael Johnson and Ramsey Saleem and John Aitchison and Lee Hood and Lasky, {Stephen R.}",

note = "Publisher Copyright: {\textcopyright} 2004, Lausted et al.; licensee BioMed Central Ltd.",

year = "2004",

month = jul,

doi = "10.1186/gb-2004-5-8-r58",

language = "English (US)",

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AU - Lausted, Christopher

AU - Dahl, Timothy

AU - Warren, Charles

AU - King, Kimberly

AU - Smith, Kimberly

AU - Johnson, Michael

AU - Saleem, Ramsey

AU - Aitchison, John

AU - Hood, Lee

AU - Lasky, Stephen R.

PY - 2004/7

Y1 - 2004/7

N2 - DNA arrays are valuable tools in molecular biology laboratories. Their rapid acceptance was aided by the release of plans for a pin-spotting microarrayer by researchers at Stanford. Inkjet microarraying is a flexible, complementary technique that allows the synthesis of arrays of any oligonucleotide sequences de novo. We describe here an open-source inkjet arrayer capable of rapidly producing sets of unique 9,800-feature arrays.

AB - DNA arrays are valuable tools in molecular biology laboratories. Their rapid acceptance was aided by the release of plans for a pin-spotting microarrayer by researchers at Stanford. Inkjet microarraying is a flexible, complementary technique that allows the synthesis of arrays of any oligonucleotide sequences de novo. We describe here an open-source inkjet arrayer capable of rapidly producing sets of unique 9,800-feature arrays.

KW - Additional Data File

KW - Oligonucleotide Synthesis

KW - Phosphoramidite

KW - Print Head

KW - Propylene Carbonate

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ER -

POSaM: a fast, flexible, open-source, inkjet oligonucleotide synthesizer and microarrayer

Abstract

Keywords

ASJC Scopus subject areas

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