Polymeric microspheres as stabilizing anchors for oligonucleotide delivery to dendritic cells

Jeffrey R. Kovacs; Ying Zheng; Hongmei Shen; Wilson S. Meng

doi:10.1016/j.biomaterials.2005.04.022

Polymeric microspheres as stabilizing anchors for oligonucleotide delivery to dendritic cells

Jeffrey R. Kovacs, Ying Zheng, Hongmei Shen, Wilson S. Meng

Research output: Contribution to journal › Article › peer-review

Abstract

The aim of this study is to evaluate a novel microspheric vector for delivery of oligonucleotides (ODN) into dendritic cells (DC). A requirement of decoy-based modulation of transcriptional activities in DC is that the ODN would have to accumulate inside the cell. Using an ex vivo DC culture model, we demonstrate that anionic microspheres (MS) coated with an ornithine/histadine- based cationic peptide (O10H6) is an effective carrier of short ODN. This method does not disrupt the colloidal nature of the microspheric particles. The MS provide stabilizing effect on DNA and O10H6 complexation. Accumulation of ODN in DC is greatly enhanced with the surface modified MS. Taken together, these data demonstrate that the self assembly system of MS_O10H6 is an effective delivery vehicle for DNA-based modulation of DC functions.

Original language	English (US)
Pages (from-to)	6754-6761
Number of pages	8
Journal	Biomaterials
Volume	26
Issue number	33
DOIs	https://doi.org/10.1016/j.biomaterials.2005.04.022
State	Published - Nov 2005
Externally published	Yes

Keywords

Cationic peptide
DNA delivery
Dendritic cell
Nanoparticle
Oligonucleotide

ASJC Scopus subject areas

Bioengineering
Ceramics and Composites
Biophysics
Biomaterials
Mechanics of Materials

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10.1016/j.biomaterials.2005.04.022

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title = "Polymeric microspheres as stabilizing anchors for oligonucleotide delivery to dendritic cells",

abstract = "The aim of this study is to evaluate a novel microspheric vector for delivery of oligonucleotides (ODN) into dendritic cells (DC). A requirement of decoy-based modulation of transcriptional activities in DC is that the ODN would have to accumulate inside the cell. Using an ex vivo DC culture model, we demonstrate that anionic microspheres (MS) coated with an ornithine/histadine- based cationic peptide (O10H6) is an effective carrier of short ODN. This method does not disrupt the colloidal nature of the microspheric particles. The MS provide stabilizing effect on DNA and O10H6 complexation. Accumulation of ODN in DC is greatly enhanced with the surface modified MS. Taken together, these data demonstrate that the self assembly system of MSO10H6 is an effective delivery vehicle for DNA-based modulation of DC functions.",

keywords = "Cationic peptide, DNA delivery, Dendritic cell, Nanoparticle, Oligonucleotide",

author = "Kovacs, {Jeffrey R.} and Ying Zheng and Hongmei Shen and Meng, {Wilson S.}",

note = "Funding Information: This work was supported in part by a grant from the Noble J. Dick Foundation (WSM). We thank Erin McClelland (University of Pittsburgh Medical Center) for assistance with the flow cytometric analysis.",

year = "2005",

month = nov,

doi = "10.1016/j.biomaterials.2005.04.022",

language = "English (US)",

volume = "26",

pages = "6754--6761",

journal = "Biomaterials",

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publisher = "Elsevier Ltd",

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T1 - Polymeric microspheres as stabilizing anchors for oligonucleotide delivery to dendritic cells

AU - Kovacs, Jeffrey R.

AU - Zheng, Ying

AU - Shen, Hongmei

AU - Meng, Wilson S.

N1 - Funding Information: This work was supported in part by a grant from the Noble J. Dick Foundation (WSM). We thank Erin McClelland (University of Pittsburgh Medical Center) for assistance with the flow cytometric analysis.

PY - 2005/11

Y1 - 2005/11

N2 - The aim of this study is to evaluate a novel microspheric vector for delivery of oligonucleotides (ODN) into dendritic cells (DC). A requirement of decoy-based modulation of transcriptional activities in DC is that the ODN would have to accumulate inside the cell. Using an ex vivo DC culture model, we demonstrate that anionic microspheres (MS) coated with an ornithine/histadine- based cationic peptide (O10H6) is an effective carrier of short ODN. This method does not disrupt the colloidal nature of the microspheric particles. The MS provide stabilizing effect on DNA and O10H6 complexation. Accumulation of ODN in DC is greatly enhanced with the surface modified MS. Taken together, these data demonstrate that the self assembly system of MSO10H6 is an effective delivery vehicle for DNA-based modulation of DC functions.

AB - The aim of this study is to evaluate a novel microspheric vector for delivery of oligonucleotides (ODN) into dendritic cells (DC). A requirement of decoy-based modulation of transcriptional activities in DC is that the ODN would have to accumulate inside the cell. Using an ex vivo DC culture model, we demonstrate that anionic microspheres (MS) coated with an ornithine/histadine- based cationic peptide (O10H6) is an effective carrier of short ODN. This method does not disrupt the colloidal nature of the microspheric particles. The MS provide stabilizing effect on DNA and O10H6 complexation. Accumulation of ODN in DC is greatly enhanced with the surface modified MS. Taken together, these data demonstrate that the self assembly system of MSO10H6 is an effective delivery vehicle for DNA-based modulation of DC functions.

KW - Cationic peptide

KW - DNA delivery

KW - Dendritic cell

KW - Nanoparticle

KW - Oligonucleotide

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AN - SCOPUS:22544440614

SN - 0142-9612

VL - 26

SP - 6754

EP - 6761

JO - Biomaterials

JF - Biomaterials

IS - 33

ER -

Polymeric microspheres as stabilizing anchors for oligonucleotide delivery to dendritic cells

Abstract

Keywords

ASJC Scopus subject areas

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