Platelets induce alterations of chemotactic and adhesive properties of endothelial cells mediated through an interleukin-1-dependent mechanism. Implications for atherogenesis

Meinrad Gawaz; Korbinian Brand; Timm Dickfeld; Gisela Pogatsa-Murray; Sharon Page; Caroline Bogner; Werner Koch; Albert Schömig; Franz Josef Neumann

doi:10.1016/S0021-9150(99)00241-5

Platelets induce alterations of chemotactic and adhesive properties of endothelial cells mediated through an interleukin-1-dependent mechanism. Implications for atherogenesis

Meinrad Gawaz
, Korbinian Brand
, Timm Dickfeld
, Gisela Pogatsa-Murray
, Sharon Page
, Caroline Bogner
, Werner Koch
, Albert Schömig
, Franz Josef Neumann

Research output: Contribution to journal › Article › peer-review

196 Scopus citations

Abstract

Platelets and alterations of chemotactic and adhesive properties of endothelium play an important role in the pathophysiology of atherosclerosis. We investigated the effect of platelets on secretion of monocyte chemotactic protein-1 (MCP-1) and on surface expression of intercellular adhesion molecule-1 (ICAM-1) of cultured endothelium. Pretreatment of cultured monolayers of endothelial cells with α-thrombin-activated platelets significantly enhanced secretion of MCP-1 and ICAM-1 surface expression (P3) on the luminal aspect of endothelial monolayers and promoted α(v)β₃-mediated platelet/endothelium adhesion that could be inhibited by the antiadhesive peptides GRGDSP and c(RGDfV). We conclude that activated platelets induce significant changes in chemotactic (secretion of MCP-1) and adhesive (surface expression of ICAM-1 and α(v)β₃) properties of cultured endothelium. These findings imply a potential pathophysiological mechanism of platelets in an early stage of atherogenesis. Copyright (C) 2000 Elsevier Science Ireland Ltd.

Original language	English (US)
Pages (from-to)	75-85
Number of pages	11
Journal	Atherosclerosis
Volume	148
Issue number	1
DOIs	https://doi.org/10.1016/S0021-9150(99)00241-5
State	Published - Jan 2000
Externally published	Yes

Keywords

Atherogenesis
Endothelium
Intercellular adhesion molecule-1
Monocyte chemotactic protein-1
Nuclear factor-κB
Platelets

ASJC Scopus subject areas

Cardiology and Cardiovascular Medicine

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10.1016/S0021-9150(99)00241-5

Cite this

Gawaz, M., Brand, K., Dickfeld, T., Pogatsa-Murray, G., Page, S., Bogner, C., Koch, W., Schömig, A., & Neumann, F. J. (2000). Platelets induce alterations of chemotactic and adhesive properties of endothelial cells mediated through an interleukin-1-dependent mechanism. Implications for atherogenesis. Atherosclerosis, 148(1), 75-85. https://doi.org/10.1016/S0021-9150(99)00241-5

Gawaz, M, Brand, K, Dickfeld, T, Pogatsa-Murray, G, Page, S, Bogner, C, Koch, W, Schömig, A & Neumann, FJ 2000, 'Platelets induce alterations of chemotactic and adhesive properties of endothelial cells mediated through an interleukin-1-dependent mechanism. Implications for atherogenesis', Atherosclerosis, vol. 148, no. 1, pp. 75-85. https://doi.org/10.1016/S0021-9150(99)00241-5

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title = "Platelets induce alterations of chemotactic and adhesive properties of endothelial cells mediated through an interleukin-1-dependent mechanism. Implications for atherogenesis",

abstract = "Platelets and alterations of chemotactic and adhesive properties of endothelium play an important role in the pathophysiology of atherosclerosis. We investigated the effect of platelets on secretion of monocyte chemotactic protein-1 (MCP-1) and on surface expression of intercellular adhesion molecule-1 (ICAM-1) of cultured endothelium. Pretreatment of cultured monolayers of endothelial cells with α-thrombin-activated platelets significantly enhanced secretion of MCP-1 and ICAM-1 surface expression (P3) on the luminal aspect of endothelial monolayers and promoted α(v)β3-mediated platelet/endothelium adhesion that could be inhibited by the antiadhesive peptides GRGDSP and c(RGDfV). We conclude that activated platelets induce significant changes in chemotactic (secretion of MCP-1) and adhesive (surface expression of ICAM-1 and α(v)β3) properties of cultured endothelium. These findings imply a potential pathophysiological mechanism of platelets in an early stage of atherogenesis. Copyright (C) 2000 Elsevier Science Ireland Ltd.",

keywords = "Atherogenesis, Endothelium, Intercellular adhesion molecule-1, Monocyte chemotactic protein-1, Nuclear factor-κB, Platelets",

author = "Meinrad Gawaz and Korbinian Brand and Timm Dickfeld and Gisela Pogatsa-Murray and Sharon Page and Caroline Bogner and Werner Koch and Albert Sch{\"o}mig and Neumann, \{Franz Josef\}",

year = "2000",

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doi = "10.1016/S0021-9150(99)00241-5",

language = "English (US)",

volume = "148",

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AU - Gawaz, Meinrad

AU - Brand, Korbinian

AU - Dickfeld, Timm

AU - Pogatsa-Murray, Gisela

AU - Page, Sharon

AU - Bogner, Caroline

AU - Koch, Werner

AU - Schömig, Albert

AU - Neumann, Franz Josef

PY - 2000/1

Y1 - 2000/1

N2 - Platelets and alterations of chemotactic and adhesive properties of endothelium play an important role in the pathophysiology of atherosclerosis. We investigated the effect of platelets on secretion of monocyte chemotactic protein-1 (MCP-1) and on surface expression of intercellular adhesion molecule-1 (ICAM-1) of cultured endothelium. Pretreatment of cultured monolayers of endothelial cells with α-thrombin-activated platelets significantly enhanced secretion of MCP-1 and ICAM-1 surface expression (P3) on the luminal aspect of endothelial monolayers and promoted α(v)β3-mediated platelet/endothelium adhesion that could be inhibited by the antiadhesive peptides GRGDSP and c(RGDfV). We conclude that activated platelets induce significant changes in chemotactic (secretion of MCP-1) and adhesive (surface expression of ICAM-1 and α(v)β3) properties of cultured endothelium. These findings imply a potential pathophysiological mechanism of platelets in an early stage of atherogenesis. Copyright (C) 2000 Elsevier Science Ireland Ltd.

AB - Platelets and alterations of chemotactic and adhesive properties of endothelium play an important role in the pathophysiology of atherosclerosis. We investigated the effect of platelets on secretion of monocyte chemotactic protein-1 (MCP-1) and on surface expression of intercellular adhesion molecule-1 (ICAM-1) of cultured endothelium. Pretreatment of cultured monolayers of endothelial cells with α-thrombin-activated platelets significantly enhanced secretion of MCP-1 and ICAM-1 surface expression (P3) on the luminal aspect of endothelial monolayers and promoted α(v)β3-mediated platelet/endothelium adhesion that could be inhibited by the antiadhesive peptides GRGDSP and c(RGDfV). We conclude that activated platelets induce significant changes in chemotactic (secretion of MCP-1) and adhesive (surface expression of ICAM-1 and α(v)β3) properties of cultured endothelium. These findings imply a potential pathophysiological mechanism of platelets in an early stage of atherogenesis. Copyright (C) 2000 Elsevier Science Ireland Ltd.

KW - Atherogenesis

KW - Endothelium

KW - Intercellular adhesion molecule-1

KW - Monocyte chemotactic protein-1

KW - Nuclear factor-κB

KW - Platelets

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Platelets induce alterations of chemotactic and adhesive properties of endothelial cells mediated through an interleukin-1-dependent mechanism. Implications for atherogenesis

Abstract

Keywords

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