Plasmodium yoelii: Axenic development of the parasite mosquito stages

Johanna M. Porter-Kelley, Rhoel R. Dinglasan, Uzma Alam, George A. Ndeta, John B. Sacci, Abdu F. Azad

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


Study of the parasite mosquito stages of Plasmodium and its use in the production of sporozoite vaccines against malaria has been hampered by the technical difficulties of in vitro development. Here, we show the complete axenic development of the parasite mosquito stages of Plasmodium yoelii. While we demonstrate that matrigel is not required for parasite development, soluble factors produced and secreted by Drosophila melanogaster S2 cells appear to be crucial for the ookinete to oocyst transition. Parasites cultured axenically are both morphologically and biologically similar to mosquito-derived ookinetes, oocysts, and sporozoites. Axenically derived sporozoites were capable of producing an infection in mice as determined by RT-PCR; however, the parasitemia was significantly much less than that produced by mosquito-derived sporozoites. Our cell free system for development of the mosquito stages of P. yoelii provides a simplified approach to generate sporozoites that may be for biological assays and genetic manipulations.

Original languageEnglish (US)
Pages (from-to)99-108
Number of pages10
JournalExperimental Parasitology
Issue number2
StatePublished - Feb 2006
Externally publishedYes


  • Axenic
  • DAPI,4′,6-diamidino-2-phenylindole
  • DNA, deoxyribonucleic acid
  • ELISA, enzyme-linked immunosorbent assay
  • EMEM
  • Malaria
  • Parasite mosquito stages
  • Plasmodium yoelii
  • cDNA, complementary deoxyribonucleic acid
  • cs, circumsporozoite protein

ASJC Scopus subject areas

  • Parasitology
  • Immunology
  • Infectious Diseases


Dive into the research topics of 'Plasmodium yoelii: Axenic development of the parasite mosquito stages'. Together they form a unique fingerprint.

Cite this