TY - JOUR
T1 - Plasmodium falciparum
T2 - Detection of a novel asparagine-rich protein on the surface of sporozoite
AU - Zhang, Kunyan
AU - Fujioka, Hisashi
AU - Aikawa, Masamichi
AU - Kumar, Nirbhay
PY - 1999/9
Y1 - 1999/9
N2 - We had previously cloned and characterized a gene for a novel asparagine-rich protein from P falciparum (PfARP), a target of natural human immune response. The antibodies to PfARP were localized to the surface of parasitized red blood cells and reacted with intracellular components in all erythrocytic asexual and sexual stages of the parasite. We here describe reactivity of antibodies against this novel PfARP on the surface of mosquito stage sporozoite of P. falciparum by indirect immunofluorescence assay and immunoelectron microscopy, the latter revealing a highly periodic punctate pattern of distribution of PfARP on the surface of sporozoite. These results suggest a possibility that PfARP might represent yet another sporozoite surface protein.
AB - We had previously cloned and characterized a gene for a novel asparagine-rich protein from P falciparum (PfARP), a target of natural human immune response. The antibodies to PfARP were localized to the surface of parasitized red blood cells and reacted with intracellular components in all erythrocytic asexual and sexual stages of the parasite. We here describe reactivity of antibodies against this novel PfARP on the surface of mosquito stage sporozoite of P. falciparum by indirect immunofluorescence assay and immunoelectron microscopy, the latter revealing a highly periodic punctate pattern of distribution of PfARP on the surface of sporozoite. These results suggest a possibility that PfARP might represent yet another sporozoite surface protein.
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U2 - 10.1006/expr.1999.4435
DO - 10.1006/expr.1999.4435
M3 - Article
C2 - 10464033
AN - SCOPUS:0032844809
SN - 0014-4894
VL - 93
SP - 1
EP - 6
JO - Experimental Parasitology
JF - Experimental Parasitology
IS - 1
ER -