TY - JOUR
T1 - PiUS (Pi uptake stimulator) is an inositol hexakisphosphate kinase
AU - Schell, Michael J.
AU - Letcher, Andrew J.
AU - Brearley, Charles A.
AU - Biber, Jürg
AU - Murer, Heini
AU - Irvine, Robin F.
N1 - Funding Information:
M.J.S. is a Hitchings-Elion Fellow, C.A.B. is a BBSRC Advanced Research Fellow, A.J.L. is supported by the Wellcome Trust, H.M. and J.B. by the Swiss National Science Foundation and R.F.I. by the Royal Society.
PY - 1999/11/19
Y1 - 1999/11/19
N2 - A cDNA cloned from its ability to stimulate inorganic phosphate uptake in Xenopus oocytes (phosphate uptake stimulator (PiUS)) shows significant similarity with inositol 1,4,5-trisphosphate 3-kinase. However, the expressed PiUS protein showed no detectable activity against inositol 1,4,5-trisphosphate, nor the 1,3,4,5- or 3,4,5,6-isomers of inositol tetrakisphosphate, whereas it was very active in converting inositol hexakisphosphate (InsP6) to inositol heptakisphosphate (InsP7). PiUS is a member of a family of enzymes found in many eukaryotes and we discuss the implications of this for the functions of InsP7 and for the evolution of inositol phosphate kinases. Copyright (C) 1999 Federation of European Biochemical Societies.
AB - A cDNA cloned from its ability to stimulate inorganic phosphate uptake in Xenopus oocytes (phosphate uptake stimulator (PiUS)) shows significant similarity with inositol 1,4,5-trisphosphate 3-kinase. However, the expressed PiUS protein showed no detectable activity against inositol 1,4,5-trisphosphate, nor the 1,3,4,5- or 3,4,5,6-isomers of inositol tetrakisphosphate, whereas it was very active in converting inositol hexakisphosphate (InsP6) to inositol heptakisphosphate (InsP7). PiUS is a member of a family of enzymes found in many eukaryotes and we discuss the implications of this for the functions of InsP7 and for the evolution of inositol phosphate kinases. Copyright (C) 1999 Federation of European Biochemical Societies.
KW - Inositol
KW - Inositol hexakisphosphate kinase
KW - Inositol trisphosphate kinase
KW - Phosphate uptake
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U2 - 10.1016/S0014-5793(99)01462-3
DO - 10.1016/S0014-5793(99)01462-3
M3 - Article
C2 - 10567691
AN - SCOPUS:0032744412
SN - 0014-5793
VL - 461
SP - 169
EP - 172
JO - FEBS Letters
JF - FEBS Letters
IS - 3
ER -