PiUS (Pi uptake stimulator) is an inositol hexakisphosphate kinase

Michael J. Schell; Andrew J. Letcher; Charles A. Brearley; Jürg Biber; Heini Murer; Robin F. Irvine

doi:10.1016/S0014-5793(99)01462-3

PiUS (Pi uptake stimulator) is an inositol hexakisphosphate kinase

Michael J. Schell, Andrew J. Letcher, Charles A. Brearley, Jürg Biber, Heini Murer, Robin F. Irvine

Research output: Contribution to journal › Article › peer-review

67 Scopus citations

Abstract

A cDNA cloned from its ability to stimulate inorganic phosphate uptake in Xenopus oocytes (phosphate uptake stimulator (PiUS)) shows significant similarity with inositol 1,4,5-trisphosphate 3-kinase. However, the expressed PiUS protein showed no detectable activity against inositol 1,4,5-trisphosphate, nor the 1,3,4,5- or 3,4,5,6-isomers of inositol tetrakisphosphate, whereas it was very active in converting inositol hexakisphosphate (InsP₆) to inositol heptakisphosphate (InsP₇). PiUS is a member of a family of enzymes found in many eukaryotes and we discuss the implications of this for the functions of InsP₇ and for the evolution of inositol phosphate kinases. Copyright (C) 1999 Federation of European Biochemical Societies.

Original language	English (US)
Pages (from-to)	169-172
Number of pages	4
Journal	FEBS Letters
Volume	461
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(99)01462-3
State	Published - Nov 19 1999
Externally published	Yes

Keywords

Inositol
Inositol hexakisphosphate kinase
Inositol trisphosphate kinase
Phosphate uptake

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(99)01462-3

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title = "PiUS (Pi uptake stimulator) is an inositol hexakisphosphate kinase",

abstract = "A cDNA cloned from its ability to stimulate inorganic phosphate uptake in Xenopus oocytes (phosphate uptake stimulator (PiUS)) shows significant similarity with inositol 1,4,5-trisphosphate 3-kinase. However, the expressed PiUS protein showed no detectable activity against inositol 1,4,5-trisphosphate, nor the 1,3,4,5- or 3,4,5,6-isomers of inositol tetrakisphosphate, whereas it was very active in converting inositol hexakisphosphate (InsP6) to inositol heptakisphosphate (InsP7). PiUS is a member of a family of enzymes found in many eukaryotes and we discuss the implications of this for the functions of InsP7 and for the evolution of inositol phosphate kinases. Copyright (C) 1999 Federation of European Biochemical Societies.",

keywords = "Inositol, Inositol hexakisphosphate kinase, Inositol trisphosphate kinase, Phosphate uptake",

author = "Schell, {Michael J.} and Letcher, {Andrew J.} and Brearley, {Charles A.} and J{\"u}rg Biber and Heini Murer and Irvine, {Robin F.}",

note = "Funding Information: M.J.S. is a Hitchings-Elion Fellow, C.A.B. is a BBSRC Advanced Research Fellow, A.J.L. is supported by the Wellcome Trust, H.M. and J.B. by the Swiss National Science Foundation and R.F.I. by the Royal Society.",

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doi = "10.1016/S0014-5793(99)01462-3",

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AU - Schell, Michael J.

AU - Letcher, Andrew J.

AU - Brearley, Charles A.

AU - Biber, Jürg

AU - Murer, Heini

AU - Irvine, Robin F.

N1 - Funding Information: M.J.S. is a Hitchings-Elion Fellow, C.A.B. is a BBSRC Advanced Research Fellow, A.J.L. is supported by the Wellcome Trust, H.M. and J.B. by the Swiss National Science Foundation and R.F.I. by the Royal Society.

PY - 1999/11/19

Y1 - 1999/11/19

N2 - A cDNA cloned from its ability to stimulate inorganic phosphate uptake in Xenopus oocytes (phosphate uptake stimulator (PiUS)) shows significant similarity with inositol 1,4,5-trisphosphate 3-kinase. However, the expressed PiUS protein showed no detectable activity against inositol 1,4,5-trisphosphate, nor the 1,3,4,5- or 3,4,5,6-isomers of inositol tetrakisphosphate, whereas it was very active in converting inositol hexakisphosphate (InsP6) to inositol heptakisphosphate (InsP7). PiUS is a member of a family of enzymes found in many eukaryotes and we discuss the implications of this for the functions of InsP7 and for the evolution of inositol phosphate kinases. Copyright (C) 1999 Federation of European Biochemical Societies.

AB - A cDNA cloned from its ability to stimulate inorganic phosphate uptake in Xenopus oocytes (phosphate uptake stimulator (PiUS)) shows significant similarity with inositol 1,4,5-trisphosphate 3-kinase. However, the expressed PiUS protein showed no detectable activity against inositol 1,4,5-trisphosphate, nor the 1,3,4,5- or 3,4,5,6-isomers of inositol tetrakisphosphate, whereas it was very active in converting inositol hexakisphosphate (InsP6) to inositol heptakisphosphate (InsP7). PiUS is a member of a family of enzymes found in many eukaryotes and we discuss the implications of this for the functions of InsP7 and for the evolution of inositol phosphate kinases. Copyright (C) 1999 Federation of European Biochemical Societies.

KW - Inositol

KW - Inositol hexakisphosphate kinase

KW - Inositol trisphosphate kinase

KW - Phosphate uptake

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ER -

PiUS (Pi uptake stimulator) is an inositol hexakisphosphate kinase

Abstract

Keywords

ASJC Scopus subject areas

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