TY - JOUR
T1 - Phospholipase C-mediated calcium signalling is required for fungal development and pathogenicity in Magnaporthe oryzae
AU - Rho, Hee Sool
AU - Jeon, Junhyun
AU - Lee, Yong Hwan
PY - 2009/5
Y1 - 2009/5
N2 - Calcium signalling has profound implications in the fungal infection of plants and animals, during which a series of physiological and morphological transitions are required. In this article, using a model fungal pathogen, Magnaporthe oryzae, we demonstrate that the regulation of the intracellular calcium concentration (Ca2+int) is essential for fungal development and pathogenesis. Imaging of Ca2+int showed that infection-specific morphogenesis is highly correlated with the spatiotemporal regulation of calcium flux. Deletion of the fungal phospholipase C gene (M. oryzae phospholipase C 1, MoPLC1) suppressed calcium flux, resulting in a fungus defective in developmental steps, including appressorium formation and pathogenicity. Surprisingly, the PLC-δ1 gene of mouse was able to functionally substitute for MoPLC1 by restoring the calcium flux, suggesting the evolutionary conservation of the phospholipase C-mediated regulation of calcium flux. Our results reveal that MoPLC1 is a conserved modulator of calcium flux that is essential for the regulation of key steps in fungal development and pathogenesis.
AB - Calcium signalling has profound implications in the fungal infection of plants and animals, during which a series of physiological and morphological transitions are required. In this article, using a model fungal pathogen, Magnaporthe oryzae, we demonstrate that the regulation of the intracellular calcium concentration (Ca2+int) is essential for fungal development and pathogenesis. Imaging of Ca2+int showed that infection-specific morphogenesis is highly correlated with the spatiotemporal regulation of calcium flux. Deletion of the fungal phospholipase C gene (M. oryzae phospholipase C 1, MoPLC1) suppressed calcium flux, resulting in a fungus defective in developmental steps, including appressorium formation and pathogenicity. Surprisingly, the PLC-δ1 gene of mouse was able to functionally substitute for MoPLC1 by restoring the calcium flux, suggesting the evolutionary conservation of the phospholipase C-mediated regulation of calcium flux. Our results reveal that MoPLC1 is a conserved modulator of calcium flux that is essential for the regulation of key steps in fungal development and pathogenesis.
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U2 - 10.1111/j.1364-3703.2009.00536.x
DO - 10.1111/j.1364-3703.2009.00536.x
M3 - Article
C2 - 19400837
AN - SCOPUS:64949084885
SN - 1464-6722
VL - 10
SP - 337
EP - 346
JO - Molecular Plant Pathology
JF - Molecular Plant Pathology
IS - 3
ER -