Abstract
Summary. Described is an alternative procedure for the phenotyping of pig α1B‐glycoprotein (PO2) and haemopexin. The procedure is based on the separation of serum samples by horizontal polyacrylamide gel electrophoresis, passive blotting onto a nitrocellulose (NC) sheet, and immunochemical detection using a mixture of a primary antibody (rabbit anti‐pig α1B or anti‐pig haemopexin) and a peroxidase‐labelled secondary antibody. Several NC copies can be obtained from a single gel and these can be developed with different monospecific antisera.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 295-298 |
| Number of pages | 4 |
| Journal | Animal Genetics |
| Volume | 20 |
| Issue number | 4 |
| DOIs | |
| State | Published - Aug 1989 |
| Externally published | Yes |
Keywords
- 1D electrophoresis
- AIB and HPX phenotyping
- haemopexin (HPX)
- passive immunoblotting
- pig
- α B‐glycoprotein (PO2)
ASJC Scopus subject areas
- Animal Science and Zoology
- Genetics
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