Phenotypic heterogeneity within clones of fetal human cells

S. F. Gilbert, J. Axelman, B. R. Migeon

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

The heterogeneity of cell morphology characteristics of some colonies of human fetal kidney and amniotic fluid cells have been analyzed by biochemical and cell-cloning techniques. All the presumed subclones derived from dimorphic colonies were initially epithelioid, but some cells became fibroblastlike as the cell density increased. To determine if the observed heterogeneity occurred within clonal populations of cells, the authors determined the isozyme phenotype of dimers from renal cells heterozygous for glucose-6-phosphate dehydrogenase (G6PD). Colonies showing mixed cellular morphology expressed only a single G6PD isoenzyme, thus revealing their single-cell origin. The authors results indicate that cell morphology is influenced by the cellular density within the clone, and that a single human renal cell in vitro can yield progeny of two morphological types.

Original languageEnglish (US)
Pages (from-to)950-956
Number of pages7
JournalAmerican journal of human genetics
Volume33
Issue number6
StatePublished - Dec 1 1981
Externally publishedYes

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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